The investigation to the effect of radiosensitive enhancement of nimesulide in lung cancer cell line

SONG Lin; et al

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The investigation to the effect of radiosensitive enhancement of nimesulide in lung cancer cell line

更新时间：2024-01-17

- The investigation to the effect of radiosensitive enhancement of nimesulide in lung cancer cell line
- Journal of Chongqing Medical University Issue 7, Pages: 773-776(2008)
- 作者机构：
  
  重庆医科大学基础医学院放射医学教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R734.2
- Published：2008，
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[1]宋琳,李少林,刘芳欣,王树斌,柏玮,彭志平.尼美舒利对A549细胞株放疗增敏作用的观察[J].重庆医科大学学报,2008(07):773-776.

SONG Lin, et al. The investigation to the effect of radiosensitive enhancement of nimesulide in lung cancer cell line. [J]. Journal of Chongqing Medical University (7):773-776(2008)
[1]宋琳,李少林,刘芳欣,王树斌,柏玮,彭志平.尼美舒利对A549细胞株放疗增敏作用的观察[J].重庆医科大学学报,2008(07):773-776. DOI：

SONG Lin, et al. The investigation to the effect of radiosensitive enhancement of nimesulide in lung cancer cell line. [J]. Journal of Chongqing Medical University (7):773-776(2008) DOI：

摘要

目的:观察尼美舒利（Nimesulide）作为环氧化酶2（Cyclooxygenase-2

COX-2）抑制剂对肺腺癌细胞株A549的放射增敏作用并探讨其作用机制。方法:对数期生长的A549细胞株

按照实验要求分为对照组、药物组、照射组及实验组

流式细胞仪检测各组肿瘤细胞凋亡率及对Bcl-2、Bax蛋白表达的影响;克隆形成实验检测放射增敏作用。结果:小剂量（75mmol/L）尼美舒利与射线照射联合应用能够上调Bax的蛋白表达

同时抑制Bcl-2蛋白表达

明显增加肿瘤细胞的凋亡

具有统计学差别。结论:COX-2抑制剂尼美舒利对人肺腺癌A549细胞株具有明显的放疗增敏作用。

Abstract

Objective:To investigate the enhancement of radiosensition of COX-2 inhibitor Nimesulide on lung cancer cell line (A549) in vitro and try to reaserch the possible radiosensitizing mechanisms. Methods:A549 cell line which is in logarithmic phase was devided into four group:control group

drug group

radiation group and case group. The apoptosis and the expression in protein of Bcl-2 and Bax were measured by flow cytometry(FCM). The enhancement of radiosensitising effect was detect by clone formation experiment. Results:Low dose of Nimesulide combined with X-ray can induce the up-regulation of the protein of Bax and inhibit the expression of the protein of Bcl-2

that increased the apoptosis of tumour cell. The difference is significant. Conclusion:It is suggested that Cox-2 inhibitor-Nimesulide enhance the radiosensitivity to the cell line A549 of adenocarcinoma of lung.

关键词

环氧化酶2选择性抑制剂放射敏感性肺腺癌凋亡X线

Keywords

Selective cyclooxygene-2 inhibitorradiosensitivityLung cancerapoptosisX-ray

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Related Institution

Department of Radiation Medicine,College of Basic Medicine,Chongqing Medical University

重庆医科大学基础医学院放射医学教研室

Department of Gastrointestinal Surgery，The Third Affiliated Hospital of Zunyi Medical University/ The First People’s Hospital of Zunyi

Department of Critical Care Medicine,General Hospital of Ningxia Medical University

Department of Respiratory Medicine,Cangzhou People's Hospital

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