Haemodialysis patients sera induced angiogenesis in vitro by oxidative stress

WANG Xi-chao; et al

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Haemodialysis patients sera induced angiogenesis in vitro by oxidative stress

更新时间：2024-01-17

- Haemodialysis patients sera induced angiogenesis in vitro by oxidative stress
- Journal of Chongqing Medical University Issue 6, Pages: 661-664(2008)
- 作者机构：
  
  1. 重庆医科大学附属第一医院肾内科
  2. 重庆医科大学公共卫生学院流行病学教研室
  3. 重庆医科大学附属第一医院内分泌科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R459.5
- Published：2008，
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[1]王喜超,甘华,杜晓刚,李正荣,巫江,李革,杜鹃.氧化应激促进维持性血透患者血清体外诱导血管新生[J].重庆医科大学学报,2008(06):661-664+677.

WANG Xi-chao, et al. Haemodialysis patients sera induced angiogenesis in vitro by oxidative stress. [J]. Journal of Chongqing Medical University (6):661-664(2008)
[1]王喜超,甘华,杜晓刚,李正荣,巫江,李革,杜鹃.氧化应激促进维持性血透患者血清体外诱导血管新生[J].重庆医科大学学报,2008(06):661-664+677. DOI：

WANG Xi-chao, et al. Haemodialysis patients sera induced angiogenesis in vitro by oxidative stress. [J]. Journal of Chongqing Medical University (6):661-664(2008) DOI：

摘要

目的:探讨维持性血液透析患者的氧化应激状态诱导血管新生。方法:11例血液透析患者口服VitE400mg/d

连续服用6周（干预组）

另外11例透析患者常规治疗（非干预组）。用10%干预组患者血清培养基和10%非干预组患者血清培养基培养人脐静脉内皮细胞（Human umbilical vein endothelial cells

HUVEC）。TBA法检测血浆丙二醛（Malondialdehyde

MDA）水平

倒置显微镜下观察血管样结构形成

MTT法检测细胞增殖

用Transwell小室测定细胞迁移能力

比色法检测细胞内活性氧产生。结果:在干预组

治疗后血浆MDA水平低于治疗前

用非干预组血清细胞培养30h后

有血管样结构形成

平均面积（1438±112）μm2

细胞迁移及增殖能力增强

细胞内活性氧产生增加。结论:维持性血透患者血清能够诱导HUVEC血管样结构形成

氧化应激状态是其重要机制。

Abstract

Objective:To prove that haemodialysis patients sera medium can induce angiogenesis by oxidative stress. Method: Eleven haemodialysis patients were administered 400mg/d of vitamin E for 6 weeks per os (treated group)

and another eleven haemodialysis patients were treated regularly(non-treated group).HUVEC were cultured in 10% treated patients sera medium and 10% non-treated patients sera medium respectively. The plasma level of MDA was detected by TBA method. Angiogenesis were observed under inverted microscope

cell proliferation examinded by MTT

cell migration measured in transwell

and ROS investigated by colorimetry. Results:In treated group

the plasma level of MDA was lower after treatment.There were tube formation(average area as 1 438μm2±112μm2) and more cell proliferation and migration after cells were cultured in non- treated haemodialysis patients sera.The production of ROS was higher than that of cells exposed to treated patients sera medium.Conclusion:Haemodialysis patients sera medium can induce angiogenesis and oxidative stress is the important mechanism.

关键词

血管形成内皮细胞血液透析氧化应激

Keywords

angiogenesisendothelial cellsHaemodialysisoxidative stress

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