Observation on protection by immunization with mix recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine of echinococcus multilocularis

JIANG Guang-qiong; et al

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Observation on protection by immunization with mix recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine of echinococcus multilocularis

更新时间：2024-01-17

- Observation on protection by immunization with mix recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine of echinococcus multilocularis
- Journal of Chongqing Medical University Issue 7, Pages: 780-783(2008)
- 作者机构：
  
  重庆医科大学附属第一医院传染病寄生虫病研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC： R392
- Published：2008，
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[1]蒋光琼,李文桂,王鸿,朱佑明.多房棘球绦虫混合重组BCG-EmⅡ/3和BCG-Em14-3-3疫苗诱导的保护力观察[J].重庆医科大学学报,2008(07):780-783.

JIANG Guang-qiong, et al. Observation on protection by immunization with mix recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine of echinococcus multilocularis. [J]. Journal of Chongqing Medical University (7):780-783(2008)
[1]蒋光琼,李文桂,王鸿,朱佑明.多房棘球绦虫混合重组BCG-EmⅡ/3和BCG-Em14-3-3疫苗诱导的保护力观察[J].重庆医科大学学报,2008(07):780-783. DOI：

JIANG Guang-qiong, et al. Observation on protection by immunization with mix recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine of echinococcus multilocularis. [J]. Journal of Chongqing Medical University (7):780-783(2008) DOI：

摘要

目的:探讨多房棘球绦虫（Echinococcus multilocularis

Em）混合重组BCG-EmⅡ/3和BCG-Em14-3-3疫苗免疫小鼠后对Em原头节攻击感染的保护性作用。方法:将混合重组BCG疫苗采用皮下注射和鼻腔内接种分别免疫BALB/c鼠

免疫后8周用多房棘球绦虫原头节进行攻击感染

感染后18周剖杀小鼠

计算减蚴率

测定血清中IgG及其亚类和IgE水平

同时设有空载体、BCG和PBS对照。结果:混合疫苗接种组的减蚴率为45.29%～76.47%

血清IgG、IgG2a、IgG2b和IgE水平明显升高

IgG1和IgG3显著降低。结论:多房棘球绦虫混合重组BCG-EmⅡ/3和BCG-Em14-3-3疫苗鼻腔内接种是一种较好的途径

IgG、IgG2a、IgG2b和IgE在疫苗诱导的保护力中起重要作用。

Abstract

Objective:To investigate protection by immunization with mix recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine of echinococcus multilocularis(Em) and against challenge with Em protoscoleces. Methods:BALB/c mice were vaccinated by mix recombinant BCG vaccines subcutaneously and intranasally respectively

challenged with Em protoscoleces 8w after vaccination and killed 18w after infection to count rate of reduced alveolar echinococcus weight and to examine the level of IgE

IgG and its subclasses. Blank vector

BCG and PBS served as control. Results:In the groups of immunization.The rate of reduced alveolar echinococcus weight was 45.29%～76.47%

the level of IgG

IgG2a

IgG2b and IgE increased apparently

that of IgG1 and IgG3 decreased remarkably. Conclusion:Intranasal vaccination with mix recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine may be a good route

IgG

IgG2a

IgG2b and IgE may play an important role in protection induced by this vaccine.

关键词

多房棘球绦虫重组BCG-EmⅡ/3疫苗重组BCG-Em14-3-3疫苗保护力

Keywords

Echinococcus multilocularisRecombinant BCG-EmⅡ/3 vaccineRecombinant BCG-Em14-3-3 vaccineProtection

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