The value of CT scan and tumor markers in sputum for early diagnosis of peripheral lung cancer

CAO Ai-hong; et al

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The value of CT scan and tumor markers in sputum for early diagnosis of peripheral lung cancer

更新时间：2024-01-17

- The value of CT scan and tumor markers in sputum for early diagnosis of peripheral lung cancer
- Journal of Chongqing Medical University Issue 10, Pages: 1193-1195(2008)
- 作者机构：
  
  1. 东南大学附属中大医院放射科
  2. 徐州医学院第二附属医院放射科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R734.2
- Published：2008，
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[1]曹爱红,王绪,石红建.周围型肺癌的早期诊断—CT扫描结合痰液肿瘤标志物检测[J].重庆医科大学学报,2008(10):1193-1195.

CAO Ai-hong, et al. The value of CT scan and tumor markers in sputum for early diagnosis of peripheral lung cancer. [J]. Journal of Chongqing Medical University (10):1193-1195(2008)
[1]曹爱红,王绪,石红建.周围型肺癌的早期诊断—CT扫描结合痰液肿瘤标志物检测[J].重庆医科大学学报,2008(10):1193-1195. DOI：

CAO Ai-hong, et al. The value of CT scan and tumor markers in sputum for early diagnosis of peripheral lung cancer. [J]. Journal of Chongqing Medical University (10):1193-1195(2008) DOI：

摘要

目的:研究CT扫描与肺活检后痰液脱落细胞的端粒酶活性和p16甲基化检测对早期周围型肺癌的诊断意义。方法:用PCR-TRAP-ELISA半定量及PCR-TRAP银染定性法、甲基化相关的PCR法

前瞻性检测55例经CT扫描而发现的肺部孤立性结节（直径≤30mm）、并疑诊为早期周围型肺癌（T1N0M0）

行CT导向肺活检（纤维支气管镜肺活检33例

经皮肺针刺活检22例）后24h内痰液脱落细胞端粒酶活性及p16甲基化状态

并将检测结果与组织病理进行对照研究。结果:CT扫描对周围型肺癌诊断的敏感性为100%

但特异性只有61.8%（34/55）。端粒酶活性的敏感性为79.4%

特异性为90.5%

准确性为83.6%;p16甲基化的敏感性为32.4%

特异性为100%

准确性为58.2%;三者联合检测的敏感性为86.1%

特异性为90.5%

准确性为87.3%。对照组端粒酶阳性率9.5%（2/21）

无1例检测到p16甲基化。结论:CT扫描与端粒酶活性、p16甲基化联合检测可弥补痰液的细胞学检查的不足

提高肺癌痰检的敏感性

有助于周围型肺癌早期诊断和肺部孤立性结节的鉴别诊断。

Abstract

Objective:To investigate the diagnostic value of telomerase activity and p16 gene methylation from exfoliated cells of sputum in 55 cases of solitary nodules and of being suspected of early peripheral lung cancer(T1N0M0).Methods:The sputum specimens from 34 cases of cancer nodes and 21 cases of benign lung lesion were detected for telomerase activity and p16 gene methylation by methylation analysis.Results:The qualitative diagnosis accuracy of CT scan was 61.8%(34/55) for peripheral lung cancer.Telomerase activity was positive in 29 cases:Sensitivity was 79.4%;Specificity was 90.5%;and accuracy was 83.6%.p16 gene methylation was in 11 cases:Sensitivity was 32.4%;specificity was 100%

and veracity was 58.2%.The sensitivity was increased to 86.1% by the combination of telomerase activity and p16 gene methylatiion.Conclusion:The results suggest that combining CT scan with telomerase activity and p16 gene methylation detection in sputum for patients with lung cancer may enhance the diagnostic value of conventional cytology.It is a promising approach to early diagnosis of lung cancer and massive screening in terms of its rapidity

economy and simplicity.

关键词

肺肿瘤痰液端粒酶p16甲基化

Keywords

lung neoplasmsputumTelomerase activityp16 gene methylation

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