The differential expression of VEGF165b in the renal cell carcinoma and normal renal tissues and its clinical significance

JIANG Cui-ping; et al

您当前的位置：

首页 >

文章列表页 >

The differential expression of VEGF165b in the renal cell carcinoma and normal renal tissues and its clinical significance

更新时间：2024-01-17

- The differential expression of VEGF165b in the renal cell carcinoma and normal renal tissues and its clinical significance
- Journal of Chongqing Medical University Vol. 34, Issue 3, Pages: 281-284(2009)
- 作者机构：
  
  重庆医科大学附属第一医院泌尿外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.11
- Published：2009，
扫描看全文
[1]姜翠苹,王德林,彭波.肾细胞癌组织与正常组织中VEGF165b的差异表达及其意义[J].重庆医科大学学报,2009,34(03):281-284.

JIANG Cui-ping, et al. The differential expression of VEGF165b in the renal cell carcinoma and normal renal tissues and its clinical significance. [J]. Journal of Chongqing Medical University 34(3):281-284(2009)
[1]姜翠苹,王德林,彭波.肾细胞癌组织与正常组织中VEGF165b的差异表达及其意义[J].重庆医科大学学报,2009,34(03):281-284. DOI：

JIANG Cui-ping, et al. The differential expression of VEGF165b in the renal cell carcinoma and normal renal tissues and its clinical significance. [J]. Journal of Chongqing Medical University 34(3):281-284(2009) DOI：

摘要

目的:研究血管内皮生长因子165b（Vascular endothelial growth factor 165b

VEGF165b）在肾细胞癌（Renalcellcarcino-ma

RCC）组织和正常组织中的表达情况

初步探讨其与肾细胞癌发生发展的关系。方法:（1）用S-P免疫组化法检测30例肾细胞癌组织和29例正常肾组织中VEGF165b蛋白的表达情况;（2）用RT-PCR法分别检测32例肾细胞癌组织和30例正常肾组织中VEGF165b mRNA的表达情况。结果:（1）正常肾组织中VEGF165b蛋白表达率为96.55%（28/29）

肾细胞癌组织中VEGF165b蛋白的表达率为20.00%（6/30）

表达差异有统计学意义（P<0.05）。（2）正常肾组织中VEGF165b mRNA阳性表达率为93.33%（28/30）

肾细胞癌组织中VEGF165b mRNA的阳性表达率为18.75%（6/32）

具有统计学意义（P<0.05）。结论:VEGF165b在肾细胞癌组织中的表达低于正常肾组织的表达

这种表达差异可能与肾癌发生、发展的机制有关。

Abstract

Objective:To investigate the differential expression of VEGF165b in the renal cell carcinoma (RCC) and normal renal tissues and its role in the development of RCC. Methods: S-P immunohistochemistry was used to detect the expression of VEGF165b protein in 30 specimens of paraffin-embedded RCC tissues and 29 specimens of paraffin-embedded normal renal tissues. RT-PCR was performed to detect the expression of VEGF165b mRNA in 32 specimens of fresh RCC tissues and 30 specimens of fresh normal renal tissues. Results: Among 29 of normal renal tissues

28 specimens had positive expression of VEGF165b protein

with the positive rate of 96.55%(28/29) that was significantly higher than 20.00%(6/30)in RCC tissues(P<0.05). The positive rate of VEGF165b mRNA in normal renal tissues was 93.33%(28/30)

while that of RCC tissues was only 18.75%(6/32). The positive expression rate of VEGF165b mRNA in RCC tissues was significantly lower than that in normal renal tissues (P<0.05). Conclusion: VEGF165b might play a role in inhibiting development ofRCC. Vascular endothelial growth factor 165b

关键词

肾肿瘤VEGF165b免疫组织化学逆转录-聚合酶链反应（RT-PCR）

Keywords

KidneyneoplasmsVascular endothelial growth factor 165bimmunohistochemistryReverse transcriptase polymerase chain reaction(RT-PCR)

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Expression and significance of FOXP3 in colon cancer cells

Expression of HIF-1α and Sema4D in pancreatic carcinoma and its clinical significance

HISTOCHEMICAL AND IMMUNOHISTOCHEMI- CAL STUDY OF MAMMAKY PAGET’S DISEASE

THE EXPRESSION AND SIGMFICANCE OF HMB45,S-100 PROTEIN AND VIMENTIN IN PRIMARY MALIGNANT MELANOMA

Histopathologic examination of uterine smooth muscle tumors

Related Author

Qiu Lei

Shao Hua

Miao Yongchang

Jia Rujiang

Yin Qingchen

Wang Xiuchao

Hou Liyan

Xu Chao

Related Institution

Department of Gastrointestinal Surgery,The Second People’s Hospital of Lianyungang City

Department of General Surgery,Handan Central Hospital

Department of Pancreatic Oncology,Tianjin Medical University Cancer Institute and Hospital

重庆医科大学基础医学院病理教研室

重庆医科大学临床学院眼科

⁰