Glycogen synthase kinase-3β (GSK-3β) involved in the inhibitory effect on proliferation of lung carcinoma cells in vitro

LIU Pian; et al

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Glycogen synthase kinase-3β (GSK-3β) involved in the inhibitory effect on proliferation of lung carcinoma cells in vitro

更新时间：2024-01-17

- Glycogen synthase kinase-3β (GSK-3β) involved in the inhibitory effect on proliferation of lung carcinoma cells in vitro
- Journal of Chongqing Medical University Vol. 34, Issue 2, Pages: 174-177(2009)
- 作者机构：
  
  1. 重庆医科大学附属第二医院呼吸内科
  2. 重庆医科大学基础研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC： R734.2
- Published：2009，
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[1]刘翩,蒋幼凡,岳秀,刘晓.GSK-3β参与抑制肺癌细胞增殖的实验研究[J].重庆医科大学学报,2009,34(02):174-177.

LIU Pian, et al. Glycogen synthase kinase-3β (GSK-3β) involved in the inhibitory effect on proliferation of lung carcinoma cells in vitro. [J]. Journal of Chongqing Medical University 34(2):174-177(2009)
[1]刘翩,蒋幼凡,岳秀,刘晓.GSK-3β参与抑制肺癌细胞增殖的实验研究[J].重庆医科大学学报,2009,34(02):174-177. DOI：

LIU Pian, et al. Glycogen synthase kinase-3β (GSK-3β) involved in the inhibitory effect on proliferation of lung carcinoma cells in vitro. [J]. Journal of Chongqing Medical University 34(2):174-177(2009) DOI：

摘要

目的:探讨糖原合成酶激酶-3β（Glycogen synthase kinase-3β

GSK-3β）对肺癌A549细胞增殖的影响及可能机制。方法:MTT法检测不同浓度曲古抑菌素A（TSA）和联合GSK-3β抑制剂SB216763对A549细胞增殖的影响;Western Blot法检测各组细胞中GSK-3β和磷酸化GSK-3β（pGSK-3β）蛋白表达的变化;流式细胞仪分析细胞周期;免疫细胞化学法检测p27蛋白水平。结果:TSA以剂量依赖性抑制A549细胞生长

使细胞周期阻滞于G0/G1期

用SB216763预处理后明显减弱了TSA对A549细胞的生长抑制作用和细胞周期阻滞。TSA对细胞GSK-3β蛋白表达无明显影响

却降低了pGSK-3β蛋白水平

p27蛋白表达增加;抑制剂SB216763使pGSK-3β表达增加

下调p27蛋白水平。结论:GSK-3β参与并促进了TSA对肺癌细胞的生长抑制和细胞周期阻滞效应

其机制可能与GSK-3β对p27蛋白的调节有关。

Abstract

Objective:To explore the effect of Glycogen synthase kinase-3β(GSK-3β)on cell proliferation in human lung adenocarcinoma cell line A549 and its potential mechanisms.Methods:MTT assay was employed to evaluate the effects of trichostatin A(TSA)and pretreatment with SB216763(a specific inhibitor of GSK-3β)on the proliferation of A549 cells.Western Blot was employed to analyze the protein levels of GSK-3βand phosphorylated GSK-3β(pGSK-3β).Cell life cycle was examined by flow cytometry analysis.The immunocytochemical method was employed to analyze the protein levels of p27.Results:TSA could inhibit the proliferation of A549 cells in a dose-dependent manner and the cell cycle was arrested in G0/G1 phase in A549 cells treated with TSA.While pre-treatment of A549 cells with SB216763 attenuated TSA induced growth inhibiting and cell cycle arrest.TSA did not alter the levels of GSK-3βbut decreased pGSK-3βexpression and up-regulated p27 expression.As expected

SB216763 increased the levels of pGSK-3β

consistent with inhibition of GSK-3βactivity.Inhibition of GSK-3βactivity down regulated p27 protein level.Conclusion:GSK-3βwas involved in the inhibition of effect induced by TSA on lung carcinoma cells proliferation process.GSK-3βmay exert this effect by stabilization of p27 which may be one of the downstream molecules.

关键词

糖原合成酶激酶-3β肺癌曲古抑菌素Ap27

Keywords

glycogen synthase kinase-3βLung carcinomatrichostatin Ap27

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