Objective:To compare different detaining time of the rat model by C6 gliomas implanted and to raise the achievement ratio of the model.Methods:36 rats were divided into 3 groups according to detaining time.Group 1:The detaining time was 3min;group 2 was 5min;and group 3 was 10min.The volume of C6 cell was 10μl and injection time was 5min for each group.The tresis vulnus was sealed with bone wax.Plain scan and enhanced MRI were performed from 2 weeks to 3 weeks after operation.Statistical analysis was performed by using Fisher analysis for comparison of three data

and P values were calculated for each comparison.P≤0.05 was considered to indicate a statistically significant difference.Results:Of the thirty-six rats glioma models

sixteen rats were modeled successfully in the first time;twenty rats were modeled again by changing condition;two rats were dead for anesthetic accident;eigteen rats were planted successfully.There was a significant difference in the achivement ratio among the three groups:(0.01<P<0.05)

which showed the achievement ratio of group 3(detaining time was 10 minutes) was highest.Detaining time of microinjector was very important in the rat model by C6 gliomas implanted and longer detaining time can raise the achievement ratio of the model.