Proteomic analysis of cerebrospinal fluid from patients with Parkinson’s disease

XU Fu-rong; et al

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Proteomic analysis of cerebrospinal fluid from patients with Parkinson’s disease

更新时间：2024-01-17

- Proteomic analysis of cerebrospinal fluid from patients with Parkinson’s disease
- Journal of Chongqing Medical University Issue S1, Pages: 63-66(2008)
- 作者机构：
  
  重庆医科大学附属第一医院神经内科重庆市神经病学重点实验室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R742.5
- Published：2008，
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[1]徐芙蓉,江思德,彭国光.帕金森病患者脑脊液差异蛋白组学研究[J].重庆医科大学学报,2008(S1):63-66.

XU Fu-rong, et al. Proteomic analysis of cerebrospinal fluid from patients with Parkinson’s disease. [J]. Journal of Chongqing Medical University (S1):63-66(2008)
[1]徐芙蓉,江思德,彭国光.帕金森病患者脑脊液差异蛋白组学研究[J].重庆医科大学学报,2008(S1):63-66. DOI：

XU Fu-rong, et al. Proteomic analysis of cerebrospinal fluid from patients with Parkinson’s disease. [J]. Journal of Chongqing Medical University (S1):63-66(2008) DOI：

摘要

目的:通过比较帕金森病（Parkinson’s disease

PD）与头痛（正常对照组）患者脑脊液蛋白质谱

筛选和鉴定与帕金森病密切相关的疾病特异性蛋白（Disease-specific proteins

DSPs）

探寻帕金森病诊断的生物标志物。方法:以固相pH梯度等电聚焦（IPG-IEF）为第一向

垂直平板十二烷基磺酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）为第二向进行脑脊液蛋白质分离;图像分析软件PDQUEST8.0分析电泳图谱寻找有意义的差异蛋白点;运用MALDI-TOF-MS质谱鉴定

分析其意义。结果:通过比较帕金森病与头痛（正常对照组）患者脑脊液蛋白质谱发现3个有意义的差异蛋白质点:蛋白（质）酪氨酸磷酸酶（Protein-tyrosine phosphatase1B

PTP-1B）

载脂蛋白E（Apolipoprotein E）

泛素连接酶E3（E3 ubiquitin-protein ligase）。结论:帕金森病患者与头痛（正常对照组）患者脑脊液蛋白表达存在差异

这些差异蛋白有可能是帕金森病的DSPs

并有可能成为PD诊断的分子标志物。

Abstract

Objective:To Compare the two-dimensional gel electrophoresis(2D-PAGE)with cerebrospinal fluid(CSF)of Parkinson’s disease patients and control group(patients with headache)

and to find out the disease-specific proteins(DSPs)which are closely correlated with Parkinson’s Disease. Methods:proteins extracted from CSF were run in immobilized pH gradient isoelectic focusing(IPG-IEF)at the first dimension

and then run in vertical flat sodium dodecyl sulphate polyacrylamide gel electrophoresis(SDS-PA GE)at the second dimension. The protein spots in gels were visualized by silver staining protocol followed by gel scanning and analysised by Pdquest 8.0 software.Then differentially displayed protein spots were searched and identifiyed by MALDI-TOF-MS.Results:Three DSPs were found and they were Protein-tyrosine phosphatase 1B

Apolipoprotein E and E3 ubiquitin-protein ligase. Conclusion:There were different protein expressing in 2D-PAGE of Parkinson’s disease and control group.These proteins may be the DSPs of Parkinson’s disease and may be taked as biomarkers of Parkinson’s disease.

关键词

帕金森病脑脊液蛋白质组学生物标志物

Keywords

Parkinson’s diseasecerebrospinal fluidproteomicsbiomarker

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