Effects of ischemic reperfusion on the expression of MCP-1 and HO-1 and the cardio-protective role of erythropoietin

CHENG Xi-fu; et al

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Effects of ischemic reperfusion on the expression of MCP-1 and HO-1 and the cardio-protective role of erythropoietin

更新时间：2024-01-17

- Effects of ischemic reperfusion on the expression of MCP-1 and HO-1 and the cardio-protective role of erythropoietin
- Journal of Chongqing Medical University Issue 11, Pages: 1345-1348(2008)
- 作者机构：
  
  重庆医科大学附属第一医院心血管内科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R542.22
- Published：2008，
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[1]程希富,肖祥彬,马康华.缺血再灌注对大鼠心脏MCP-1和HO-1表达的影响及促红细胞生成素的心肌保护作用[J].重庆医科大学学报,2008(11):1345-1348.

CHENG Xi-fu, et al. Effects of ischemic reperfusion on the expression of MCP-1 and HO-1 and the cardio-protective role of erythropoietin. [J]. Journal of Chongqing Medical University (11):1345-1348(2008)
[1]程希富,肖祥彬,马康华.缺血再灌注对大鼠心脏MCP-1和HO-1表达的影响及促红细胞生成素的心肌保护作用[J].重庆医科大学学报,2008(11):1345-1348. DOI：

CHENG Xi-fu, et al. Effects of ischemic reperfusion on the expression of MCP-1 and HO-1 and the cardio-protective role of erythropoietin. [J]. Journal of Chongqing Medical University (11):1345-1348(2008) DOI：

摘要

目的:探讨缺血再灌注（Ischemic reperfusion

I-R）对单核细胞趋化蛋白-1（Monocyte chemoattractant protein-1

MCP-1）和血红素氧和酶-1（Heme oxygenase-1protein

HO-1）基因和蛋白表达的影响及促红细胞生成素（Erythropoietin

EPO）对I-R损伤的保护作用。方法:建立24只在体大鼠I-R模型

随机分成3组:假手术组（F组）、缺血再灌注组（O组）、促红细胞生成素组（E组）

缺血45min

再灌注180min

E组在手术前24h腹腔注射EPO（4000U/kg）。用免疫组织化学及逆转录聚合酶联反应（RT-PCR）分别测定3组大鼠心肌组织MCP-1和HO-1蛋白和mRNA的表达。结果:与F组比较

O及E组大鼠心肌组织内MCP-1和HO-1蛋白和mRNA表达显著增高（P<0.01）

E组心肌组织内MCP-1蛋白和mRNA表达水平介于其它2组之间（P<0.05）

而HO-1蛋白和mRNA表达水平又显著高于O组（P<0.01

P<0.05）。结论:EPO预处理可能通过增加HO-1蛋白和mRNA表达及抑制MCP-1蛋白和mRNA的表达

从而起到抗心脏I-R损伤的作用。

Abstract

Objective:To investigate the effect of ischemic reperfusion(I-R) on the expression of Monocyte chemoattractant protein-1(MCP-1)and heme oxygenase-1 protein(HO-1) and mRNA of myocardial tissue and the protective role of erythropoi- etin (EPO). Methods:Ischemic reperfusion rat model was established in the experiments.24 animals were randomly divided into sham operation(F)

I-R group(O)and EPO group(E)and subjected to 45 min of myocardial ischemia by ligation of left anteri- or descending coronary artery followed by 180 min of reperfusion.The animals in EPO group were intraperitoneal by injec- tioned with EPO(4 000 U/Kg)24 hours before the operation. The expression of MCP-1 and HO-1 protein and mRNA was de- termined by immunohistochemistry and RT-PCR. Results:Campared with F group

the expression of myocardial MCP-1 and HO-1 protein and mRNA increased significantly in O group and E group (P<0.01);The expression of MCP-1 protein and mRNA was significantly higher in E group than that in O group(P<0.05);The expression of HO-1 protein and mRNA was significantly higher in O group than that in E group(P<0.01 and P<0.05). Conclusion:The pretreatment of EPO could protect cardiomyocyte against IRI through upregulating HO-1 expression and downregulating MCP-1.

关键词

缺血再灌注心肌缺血再灌注损伤促红细胞生成素单核细胞趋化蛋白-1血红素氧和酶-1

Keywords

ischemia-reperfusionmyocardial ischemia-reperfusion injuryerythropoietinmonocyte chemoattractant protein-1Heme oxygenase-1

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