Inhibition effect of silencing EGFR gene by RNAi on tumorigenesis of xenograft tumor of ovarian cancer

LIN Yuan-hong; et al

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Inhibition effect of silencing EGFR gene by RNAi on tumorigenesis of xenograft tumor of ovarian cancer

更新时间：2024-01-17

- Inhibition effect of silencing EGFR gene by RNAi on tumorigenesis of xenograft tumor of ovarian cancer
- Journal of Chongqing Medical University Vol. 34, Issue 3, Pages: 265-268(2009)
- 作者机构：
  
  1. 重庆医科大学附属第一医院肿瘤科
  2. 重庆市肿瘤研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.31
- Published：2009，
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[1]林远洪,吴永忠,黄环,郭启帅,罗茜.RNAi沉默EGFR基因在卵巢癌裸鼠移植瘤的成瘤抑制作用[J].重庆医科大学学报,2009,34(03):265-268.

LIN Yuan-hong, et al. Inhibition effect of silencing EGFR gene by RNAi on tumorigenesis of xenograft tumor of ovarian cancer. [J]. Journal of Chongqing Medical University 34(3):265-268(2009)
[1]林远洪,吴永忠,黄环,郭启帅,罗茜.RNAi沉默EGFR基因在卵巢癌裸鼠移植瘤的成瘤抑制作用[J].重庆医科大学学报,2009,34(03):265-268. DOI：

LIN Yuan-hong, et al. Inhibition effect of silencing EGFR gene by RNAi on tumorigenesis of xenograft tumor of ovarian cancer. [J]. Journal of Chongqing Medical University 34(3):265-268(2009) DOI：

摘要

目的:探讨应用RNA干扰技术沉默EGFR基因对卵巢癌SKOV3细胞成瘤力的抑制作用。方法:构建针对EGFR序列特异性dsRNA的表达载体

用脂质体转染SKOV3细胞及G418筛选阳性克隆。建立裸鼠移植瘤模型

分为阳性干扰组、阴性对照组、空白对照组。监测瘤体积

测量瘤质量

采用RT-PCR、Western Blot、免疫组化检测移植瘤EGFR基因的表达。结果:阳性干扰组成瘤力明显降低

EGFR mRNA和蛋白的抑制率分别为78.8%和76.4%。结论:动物实验证实RNAi沉默EGFR基因可明显抑制卵巢癌的成瘤力。

Abstract

Objective: To investigate the inhibition effect of silencing EGFR gene by RNAi on tumorigenesis of ovarian cancer SKOV3 cells. Methods: The EGFR gene sequence-specific dsRNA expression vector was constructed

lipofectamine transfer SKOV3 cells and G418 were used for the selection of positive clones. Xenograft tumor models were established.The model mice were divided into positive interference group

negative control group

blank control group. The tumor volume was monitored;the tumor quality was measured;the expression of EGFR gene in the xenograft tumor was detected by RT-PCR

Western Blot and immunohistochemical. Results: The tumorigenesis of the positive interference group decreased significantly

EGFR mRNA and protein inhibition rates were 78.8% and 76.4%

respectively. Conclusion: The animal experiment was confirmed that Silencing of EGFR gene by RNAi can obviously inhibit the tumorigenesis of ovarian cancer.

关键词

EGFR基因RNA干扰卵巢癌

Keywords

IEGFRgeneRNAinterferenceOvariancancer

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Department of Orthopaedics,the Children’s Hospital,Chongqing Medical University

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