A study of drug resistance and related genes of imipenem-resistant pseudomonas aeruginosa

XU Biao; et al

您当前的位置：

首页 >

文章列表页 >

A study of drug resistance and related genes of imipenem-resistant pseudomonas aeruginosa

更新时间：2024-01-17

- A study of drug resistance and related genes of imipenem-resistant pseudomonas aeruginosa
- Journal of Chongqing Medical University Vol. 34, Issue 4, Pages: 466-468(2009)
- 作者机构：
  
  1. 重庆医科大学附属第一医院检验科
  2. 重庆市第三人民医院检验科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R450;R446.5
- Published：2009，
扫描看全文
[1]胥飚,王东升,余显书.耐亚胺培南铜绿假单胞菌的耐药性及相关基因研究[J].重庆医科大学学报,2009,34(04):466-468.

XU Biao, et al. A study of drug resistance and related genes of imipenem-resistant pseudomonas aeruginosa. [J]. Journal of Chongqing Medical University 34(4):466-468(2009)
[1]胥飚,王东升,余显书.耐亚胺培南铜绿假单胞菌的耐药性及相关基因研究[J].重庆医科大学学报,2009,34(04):466-468. DOI：

XU Biao, et al. A study of drug resistance and related genes of imipenem-resistant pseudomonas aeruginosa. [J]. Journal of Chongqing Medical University 34(4):466-468(2009) DOI：

摘要

目的:研究铜绿假单胞菌对亚胺培南的耐药机制。方法:取69株临床分离的耐亚胺培南铜绿假单胞菌（Imipenem-re-sistant pseudomon asaeruginosa

IRPA）

经K-B法检测耐药性;用PCR法对铜绿假单胞菌亚胺培南耐药相关的金属酶IMP、VIM基因和外膜蛋白OprD2基因等3种主要耐药基因进行了检测与分析;结果:69株IRPA均为多重耐药菌

对阿米卡星、头孢哌酮/舒巴坦、头孢吡肟敏感性较高;检出金属酶IMP和VIM型金属酶基因阳性的分别有2株（2/69

2.9%）和4株（4/69

5.8%）

外膜蛋白通道OprD2基因检测为缺失的有46株（46/69

66.7%）。结论:IRPA耐药情况严重。外膜蛋白通道OprD2基因缺失突变是本组铜绿假单胞菌对亚胺培南耐药的重要机制之一。

Abstract

Objective:To study the mechanisms of imipenem resistance in Pseudomonas aeruginosa.Methods:Drug resistance of 69 isolates of imipenem-resistant Pseudomonas aeruginosa(IRPA)were tested by Kirby-Bauer disk diffusion method;IMP and VIM genes of metallo-β-lactamases

and outer membrane protein D2(OprD2)gene of IRPA were detected and analyzed by PCR method.Results: All 69 isolates were multidrug resistant

and the sensitivity rates of AMK

CFS and FEP were highe.The positive rates for IMP gene and VIM gene were 2.9%(2/69)and 5.8%(4/69)

respectively.46 of 69(66.7%)isolates presented with deletion for OprD2 gene. Conclusion:The drug resistance of IRPA is a serious issue.The deletion mutation of OprD2 gene is one of important mechanisms of imipenem resistance in these tested P.aeruginosa isolates.

关键词

铜绿假单胞菌亚胺培南耐药机制基因

Keywords

Pseudomonas aeruginosaImipenemResistance mechanismgene

references

Views

117

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Cross resistance of Pseudomonas aeruginosa induced by ciprofloxacin and imipenem in vitro and in vivo

Bioinformatics analysis of differentially co-expressed genes in peripheral blood and hippocampal tissue of patients with Alzheimer’s disease

Effect of the interaction between Gly1057Asp in IRS2 gene and environment on the risk of type 2 diabetes

Expression of Bak and Bad gene in brain after global ischemia-reperfusion

Construction of a recombinant adenovirus vector containing HBX gene

Related Author

YUAN Zhe

et al

王其南

Wu Xianbo

Chen Ziting

Chen Peiliang

Song Weiqi

Gao Jian

Related Institution

重庆医科大学临床学院传染科　重庆　４０００１６

Department of Infectious Diseases,College of Clinical Medicine,Chon gqing Medical University

Department of Epidemiology，School of Public Health，Southern Medical University

Laboratory of Disorders Genes and Department of Pharmacology,Medicine School of Jishou University

⁰