Expression of cyclooxygenase-2 in human transitional cell carcinoma of bladder and its clinical significance

ZHANG Rong-gui; et al

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Expression of cyclooxygenase-2 in human transitional cell carcinoma of bladder and its clinical significance

更新时间：2024-01-17

- Expression of cyclooxygenase-2 in human transitional cell carcinoma of bladder and its clinical significance
- Journal of Chongqing Medical University Vol. 34, Issue 2, Pages: 197-199(2009)
- 作者机构：
  
  重庆医科大学附属第二医院泌尿外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.14
- Published：2009，
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[1]张荣贵,张唯力,徐光勇.人膀胱移行细胞癌组织中环氧合酶-2基因的表达及临床意义[J].重庆医科大学学报,2009,34(02):197-199.

ZHANG Rong-gui, et al. Expression of cyclooxygenase-2 in human transitional cell carcinoma of bladder and its clinical significance. [J]. Journal of Chongqing Medical University 34(2):197-199(2009)
[1]张荣贵,张唯力,徐光勇.人膀胱移行细胞癌组织中环氧合酶-2基因的表达及临床意义[J].重庆医科大学学报,2009,34(02):197-199. DOI：

ZHANG Rong-gui, et al. Expression of cyclooxygenase-2 in human transitional cell carcinoma of bladder and its clinical significance. [J]. Journal of Chongqing Medical University 34(2):197-199(2009) DOI：

摘要

目的:探索环氧合酶-2（Cyclooxygenase-2

COX-2）在膀胱移行细胞癌（Transitional cell carcinoma

TCC）组织中的表达及其意义。方法:采用免疫组化技术

对42例膀胱移行细胞癌和8例正常膀胱组织石蜡标本切片进行免疫组化染色

对阳性表达率与肿瘤分级及侵袭性间的相关性进行分析

逆转录聚合酶链反应（RT-PCR）研究膀胱癌组织中COX-2的表达。结果:COX-2蛋白的阳性表达率与膀胱移行细胞癌分级及侵袭性呈显著相关

在膀胱肿瘤组织中表达率为61.9%（26/42）

COX-2在正常膀胱组织中无表达。在Ⅰ级膀胱移行细胞癌中COX-2蛋白表达率为33.3%（4/12）

在Ⅲ级及Ⅲ级以上膀胱移行细胞癌中COX-2阳性表达率为87.5%（14/16）

Ⅰ级与Ⅲ级及Ⅲ级以上之间有显著差异（P<0.05）。COX-2在表浅及浸润膀胱癌中阳性表达率分别为30%和90.9%

统计分析其表达有显著差异（P<0.05）;RT-PCR结果显示正常膀胱组织COX-2mRNA无表达

而膀胱癌组织中可检测出COX-2mRNA的表达。结论:COX-2在高分级膀胱移行细胞癌和侵袭性膀胱移行细胞癌中阳性表达率显著上升

表明COX-2可能在膀胱移行细胞癌的形成中起重要作用

有可能成为治疗人类膀胱移行细胞癌的作用靶点。

Abstract

Objective:To explore the expression and significance of cyclooxygenase-2 in human transitional cell carcinoma of bladder.Methods:A total of 42 samples of paraffin-embedded TCC tissue and 8 normal bladder sample were stained by SP immunohistochemical method with specific anti COX-2 antibody.The correlation between the positive expression rates and grades of tumor was analyzed.RT-PCR studied the expression of COX-2 in bladder carcinoma tissue.Results:Expression rate of COX-2 protein was significantly correlated to the tumor grades and their invasiveness.Expression rate of COX-2 protein in TCC was 61.9%

statistically higher than those in the normal samples(P<0.05).Expression of COX-2 was detected 33.3%in Low-grade TCC

and 87.5%in high-grade TCC(P<0.05).Expression of COX-2 was detected 30.0%in non-invasive TCC

and 90.9% in invasive TCC(P<0.05).RT-PCR showed that the expression of COX-2 mRNA was strongly up-regulated in bladder carcinoma.Conclusion:The expression of COX-2 is enhanced in high-grade and invasive TCC

suggesting a possible role of COX-2 in the formation of TCC.COX-2 might be a biomarker and a therapeutic target in human TCC.

关键词

环氧合酶-2膀胱癌

Keywords

cyclooxygenase-2Bladder carcinoma

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