Inhibitory effect of disodium cantharidinate on human hepatoma cell line HepG<sub>2</sub> and its molecular mechanisms

GAO Wei; et al

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Inhibitory effect of disodium cantharidinate on human hepatoma cell line HepG₂ and its molecular mechanisms

更新时间：2024-01-17

- Inhibitory effect of disodium cantharidinate on human hepatoma cell line HepG₂ and its molecular mechanisms
- Journal of Chongqing Medical University Issue 11, Pages: 1356-1358(2008)
- 作者机构：
  
  重庆医科大学附属第一医院普通外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R735.7
- Published：2008，
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[1]高伟,郑军.斑蝥酸钠抑制人肝癌HepG_2细胞增殖及其机制的研究[J].重庆医科大学学报,2008(11):1356-1358.

GAO Wei, et al. Inhibitory effect of disodium cantharidinate on human hepatoma cell line HepG₂ and its molecular mechanisms. [J]. Journal of Chongqing Medical University (11):1356-1358(2008)
[1]高伟,郑军.斑蝥酸钠抑制人肝癌HepG_2细胞增殖及其机制的研究[J].重庆医科大学学报,2008(11):1356-1358. DOI：

GAO Wei, et al. Inhibitory effect of disodium cantharidinate on human hepatoma cell line HepG₂ and its molecular mechanisms. [J]. Journal of Chongqing Medical University (11):1356-1358(2008) DOI：

摘要

目的:探讨斑蝥酸钠对肝癌HepG2细胞增殖的作用及其机制。方法:肝癌HepG2细胞在不同浓度斑蝥酸钠干预后分别采用MTT法、流式细胞术、免疫细胞化学法、比色法等检测细胞增殖抑制率、细胞周期变化、Survivin蛋白表达和Caspase-3活性。结果:斑蝥素能明显抑制肝癌HepG2细胞增殖。药物干预后

细胞出现G2/M期阻滞

Survivin蛋白表达抑制

Caspase-3活性增强。结论:斑蝥酸钠可能通过其G2/M期特异性阻滞

下调Survivin表达和激活Caspase-3来抑制HepG2细胞增殖。

Abstract

Objective:To observe the inhibitory effect of disodium cantharidinate on human hepatoma cell line HepG2 and investi-gate its possible mechanisms. Methods:HepG2 cells were treated with different concentration of disodium cantharidinate

The in- hibiton of cell proliferation

cell cycle distribution

expression of Survivin and activity of Caspase-3 were respectively evaluated by MTT assay

flow cytometry

immunocytochemistry

and chromatometry. Results:Disodium cantharidinate could inhibit the proliferation of HepG2 cells. The IC50 at 24

72 h after intervention were 2.41±0.48

0.72±0.08

and 0.39±0.04 ug/ml

The cells of G2/M phase and activity of Caspase-3 were increased.the expression respectively of Survivin were inhibited after the intervention of disodium cantharidinate. Conclusion:The inhibitory effect of disodium cantharidinate on HepG2 cells may be mediated by the block of cellcycle

enhancement of Caspase-3

and inhibition of Survivin.

关键词

斑蝥酸钠肝癌细胞SurvivinCaspase-3

Keywords

Disodium cantharidinateHepatic carcinoma cellSurvivinCaspase-3

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重庆医科大学临床学院泌尿外科

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Inhibitory effect of disodium cantharidinate on human hepatoma cell line HepG2 and its molecular mechanisms

DOI：

摘要

Abstract

关键词

Keywords

references

Inhibitory effect of disodium cantharidinate on human hepatoma cell line HepG₂ and its molecular mechanisms