Identification and analysis of membrane estrogen receptor in inters titial cells of Cajal

LI Fan; et al

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Identification and analysis of membrane estrogen receptor in inters titial cells of Cajal

更新时间：2024-01-17

- Identification and analysis of membrane estrogen receptor in inters titial cells of Cajal
- Journal of Chongqing Medical University Vol. 34, Issue 5, Pages: 590-593(2009)
- 作者机构：
  
  第三军医大学大坪医院野战外科研究所普外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： Q57
- Published：2009，
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[1]李凡,黄颖,童卫东,刘宝华.小鼠肠道Cajal间质细胞膜雌激素受体的检测和分析[J].重庆医科大学学报,2009,34(05):590-593.

LI Fan, et al. Identification and analysis of membrane estrogen receptor in inters titial cells of Cajal. [J]. Journal of Chongqing Medical University 34(5):590-593(2009)
[1]李凡,黄颖,童卫东,刘宝华.小鼠肠道Cajal间质细胞膜雌激素受体的检测和分析[J].重庆医科大学学报,2009,34(05):590-593. DOI：

LI Fan, et al. Identification and analysis of membrane estrogen receptor in inters titial cells of Cajal. [J]. Journal of Chongqing Medical University 34(5):590-593(2009) DOI：

摘要

目的:检测并分析体外培养的小鼠肠道Cajal间质细胞（Interstitial cells of cajal

ICC）膜雌激素受体（Membrane estrogen receptor

mER）。方法:利用原代培养小鼠ICC

采用免疫荧光染色结合激光共聚焦显微镜技术分析ICC细胞膜雌激素结合位点;采用密度梯度离心法分离ICC细胞膜

采用同位素法、放射性配体结合分析法对mER的最大结合容量（Bmax）和平衡解离常数（kD）进行分析。结果:免疫组化结果证实体外成功分离培养小鼠肠道ICC;激光共聚焦观察显示

ICC细胞膜上存在雌激素作用位点;放射性配体结合分析及Scatchard分析证实ICC细胞膜雌激素受体的的受体最大容量（Bmax）为45.75fmol/mg protein

平衡解离常数（kD）为0.7173nmol/L。结论:ICC表达mER

该受体可能介导雌激素对ICC的快速非基因组作用。

Abstract

Objective:To detect and analyze the membrane estrogen receptor(mER)in primary cultured interstitial cells of Cajal(ICC).Methods:Interstitial Cells of Cajal(ICC)’s surface binding sites for 17-βestradiol(E2)were detected by cell-impermeant ligand using confocal microscopy.Radioligand binding assay and Scatchard software were used to analyzed the characteristics of mER.Results:Immunofluorescence shows the staining pattern of nonfixed

nonpermeabilized ICC incubated with E2BSAFITC.The radioligand binding assay were analyzed by Scatchard software

The Bmax of mER was 45.75 fmol/mg protein and the kD was 0.717 3 nmol/L.Conclusion:A form of the estrogen receptor is present within the cell membrane of ICC and maybe capable of mediating rapid effect of estrogen.

关键词

雌激素膜受体Cajal间质细胞雌激素

Keywords

EstrogenMembrane estrogen receptorInterstitial cells of Cajal

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