Biphasic responses of bone to parathyroid hormone-related protein-141 in vitro

DUAN Bing-nan; et al

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Biphasic responses of bone to parathyroid hormone-related protein-141 in vitro

更新时间：2024-01-17

- Biphasic responses of bone to parathyroid hormone-related protein-141 in vitro
- Journal of Chongqing Medical University Vol. 34, Issue 2, Pages: 148-151(2009)
- 作者机构：
  
  中南大学湘雅基础医学院组织学与胚胎学系
- 作者简介：
- 基金信息：
- DOI：
  CLC： R730.2
- Published：2009，
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[1]段炳南,邓锡云,黄河,张建湘.体外骨对甲状旁腺激素相关蛋白-141的双相反应[J].重庆医科大学学报,2009,34(02):148-151.

DUAN Bing-nan, et al. Biphasic responses of bone to parathyroid hormone-related protein-141 in vitro. [J]. Journal of Chongqing Medical University 34(2):148-151(2009)
[1]段炳南,邓锡云,黄河,张建湘.体外骨对甲状旁腺激素相关蛋白-141的双相反应[J].重庆医科大学学报,2009,34(02):148-151. DOI：

DUAN Bing-nan, et al. Biphasic responses of bone to parathyroid hormone-related protein-141 in vitro. [J]. Journal of Chongqing Medical University 34(2):148-151(2009) DOI：

摘要

目的:通过体外器官培养系统

探讨甲状旁腺激素相关蛋白（Parathyroid hormone-related protein

PTHrP）在新骨生成和骨组织破坏方面的效应。方法:将全长PTHrP（-36～+141）cDNA克隆进真核表达载体pcDNA3.1（+）

并用重组质粒pcDNA3.1-PTHrP转染LCC15-MB乳腺癌细胞

在体外器官培养系统里加不同浓度含PTHrP的条件培养上清液培养小鼠颅盖骨。通过组织学方法检测PTHrP141在新骨形成和骨组织破坏方面的效应。结果:转染的乳腺癌细胞产生的PTHrP在低剂量时诱导骨的形成

而高剂量时诱导骨的再吸。结论:PTHrP在新骨的形成和骨组织的破坏方面因其浓度的不同而具有不同效应。

Abstract

Objective:To determine the role of PTHrP in new bone formation and bone destruction and the mechanisms involved.Methods:LCC15-MB breast cancer cells were transfected with full-length PTHrP-141 cDNA

and the conditioned medium was collected and cultured with mouse calvarial bones in an in vitro organ culture system.The effects of 1PTHrP-141 on new bone formation and bone destruction were examined by histological examination.Results:PTHrP produced from transfected breast cancer cells induced new bone formation at lower doses and bone resorption at higher concentrations.Conclusion:These results provide direct evidence that PTHrP plays a biphasic role in bone remodeling depending on the concentration administered.

关键词

甲状旁腺激素相关蛋白颅盖骨成骨细胞破骨细胞

Keywords

Parathyroid hormone-related proteinCalvarial boneosteoblastosteoclast

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