Preparation and quality control of tetrandrine-doxorubicin complex liposomes

PAN Feng; et al

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Preparation and quality control of tetrandrine-doxorubicin complex liposomes

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- Preparation and quality control of tetrandrine-doxorubicin complex liposomes
- Journal of Chongqing Medical University Vol. 34, Issue 4, Pages: 459-462(2009)
- 作者机构：
  
  重庆医科大学药学院药物化学教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R283;TQ465
- Published：2009，
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[1]潘峰,胡春梅,潘黎军,王驰.粉防己碱-阿霉素复合脂质体的制备及质量控制评价[J].重庆医科大学学报,2009,34(04):459-462.

PAN Feng, et al. Preparation and quality control of tetrandrine-doxorubicin complex liposomes. [J]. Journal of Chongqing Medical University 34(4):459-462(2009)
[1]潘峰,胡春梅,潘黎军,王驰.粉防己碱-阿霉素复合脂质体的制备及质量控制评价[J].重庆医科大学学报,2009,34(04):459-462. DOI：

PAN Feng, et al. Preparation and quality control of tetrandrine-doxorubicin complex liposomes. [J]. Journal of Chongqing Medical University 34(4):459-462(2009) DOI：

摘要

目的:制备粉防己碱-阿霉素复合脂质体并对其工艺及质量进行研究与评价

为临床上逆转肿瘤的多药耐药提供新的思路。方法:采用3种不同脂质体制备方法

并以包封率为指标进行处方筛选和工艺优化。结果:确定了硫酸铵梯度法结合pH梯度法制备复合脂质体的最佳处方最佳处方为:药脂比1∶20

磷脂-胆固醇比例3∶1

外水相pH值为7.6

硫酸铵浓度为250mmol/L。以该处方制备的脂质体DOX的平均包封率为90.77%

TET的平均包封率为80.12%。结论:该处方制备的粉防己碱-阿霉素复合脂质体包封率及外观良好

在低温充氮条件下较稳定

为进一步实验奠定了基础。

Abstract

Objective:To prepare doxorubicin-tetrandrine complex liposomes for technology study and quality control.In order to provide a new idea to reverse the tumor multidrug resistance in clinic.Methods:The formulations of doxorubicin-tetrandrine complex liposomes were optimized by three different kinds of methods.And the optimum formula was selected according to the entrapment efficiency.Re- sults:The complex liposomes were prepared by(NH4)2SO4-gradient method combined with pH gradient method according to optimum recipe.The optimum recipe of DOX-TET liposome was founded as DOX-TET/EPC of 1∶10

EPC/Ch of 3∶1

pH value of 7.6

incubation temperature of 50℃

(NH4)2SO4 concentration of 250 mmol/L.Under the formulation

DOX and TET were encapsulated 90.77%and 80.12%

respectively.Conclusion:The doxorubicin-tetrandrine complex liposomes have high entrapment efficiency with fine looking

which provides basis for the further studies.

关键词

粉防己碱天然活性成分复合脂质体硫酸铵pH梯度法

Keywords

TetrandrineNatural active ingredientsComplex liposomes(NH)42SO4-pH gradient method

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