Curcumin inhibits proliferation of lung cancer cell A549 through Wnt signaling pathway

YUE Xiu; et al

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Curcumin inhibits proliferation of lung cancer cell A549 through Wnt signaling pathway

更新时间：2024-01-17

- Curcumin inhibits proliferation of lung cancer cell A549 through Wnt signaling pathway
- Journal of Chongqing Medical University Vol. 33, Issue 12, Pages: 1454-1457(2008)
- 作者机构：
  
  1. 重庆医科大学附属第二医院呼吸内科
  2. 重庆医科大学基础医学研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC： R734.2
- Published：2008，
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[1]岳秀,蒋幼凡,刘晓,刘翩.姜黄素通过Wnt信号转导通路抑制肺癌细胞A549增殖的研究[J].重庆医科大学学报,2008,33(12):1454-1457.

YUE Xiu, et al. Curcumin inhibits proliferation of lung cancer cell A549 through Wnt signaling pathway. [J]. Journal of Chongqing Medical University 33(12):1454-1457(2008)
[1]岳秀,蒋幼凡,刘晓,刘翩.姜黄素通过Wnt信号转导通路抑制肺癌细胞A549增殖的研究[J].重庆医科大学学报,2008,33(12):1454-1457. DOI：

YUE Xiu, et al. Curcumin inhibits proliferation of lung cancer cell A549 through Wnt signaling pathway. [J]. Journal of Chongqing Medical University 33(12):1454-1457(2008) DOI：

摘要

目的:从研究Wnt信号通路的关键因子-核蛋白β-catenin入手

探讨姜黄素（Curcumin

Cur）可能存在的抑制肺癌细胞增殖的分子学机制。方法:MTT法检测不同浓度的Cur对肺癌细胞A549的生长抑制情况

分析药物浓度和细胞增殖抑制率之间的关系;Western blot法检测Cur作用后

A549细胞核蛋白β-catenin含量的变化;RT-PCR方法验证Cur对细胞c-myc基因表达的影响;流式细胞仪观察经Cur作用后细胞周期的改变。结果:Cur以浓度依赖方式抑制肺癌细胞A549的增殖

该药物浓度与抑制率之间有明显的线性相关性（r=0.9333）。细胞经Cur干预48h后

核蛋白β-catenin的含量和促癌基因c-myc的表达含量均有所下降。流式细胞仪观察细胞周期的结果提示

Cur能够阻止肺癌细胞A549由G1期进入S期

提高G0/G1期细胞的百分比。结论:Cur能够抑制A549细胞胞浆内β-catenin蛋白进入胞核

阻断Wnt信号转导通路

进而抑制下游靶基因c-myc的表达

阻止肺癌细胞A549由G1期进入S期

有效抑制了A549细胞的增殖。

Abstract

Objective:To explore the possible molecular mechanism of inhibiting the proliferation of lung cancer cells by studing curcumin regulating neucleoprotein β-catenin

the key factor of Wnt signaling pathway.Methods:Non-toxic different doses of curcumin affected human lung adenocarcinoma cell A549 for 48 hours.Inhibitory effect of curcumin on proliferation of A549 cells was evaluated by MTT assay.The correclation of doses and inhibition ratio was analyzed statistically.The neucleoprotein levels of β-catenin was analyzed by Western blot.The expression level of c-myc was analyzed by reverse transcription-polymerase chain reaction(RT-PCR).The exchange of cell cycle was examined by flow cytometry(FCM).Results:Curcumin inhibited the proliferation of A549 in a concentration-dependent manne(rP<0.05)

and there was obvious linear correlation between drug concentrations and inhibition ratio(r=0.933 3).The expresson of nucleoprotein β-catenin and oncogene c-myc were all down-regulated in A549 cells(P<0.05)after treatment with curcumin.Curcumin prevented A549 cells from G1 to S phase period

and raised the percentage of G0/G1 phase cells(P<0.05).Conclusion:Curcumin could prevent β-catenin protein of A549 cells into the nucleus from the cytoplasm

block Wnt signal transduction pathway and inhibit the expression of downstream target genes c-myc.Thereby

curcimin prevents lung cancer cells from G1 phase to S phase

and effectively inhibit the proliferation of A549 cells.

关键词

姜黄素β-cateninWnt信号转导通路A549细胞

Keywords

curcuminβ-cateninWnt signaling pathwayA549 cell

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