Objective:To study the effect of astrocyte activating PKA signal pathway on the proligeration and differentiation of nerve stem cells.Methods:Cerebral cortex of 13-day embryo mice was taken and the nerve stem cells were cultured scatteringly. Astrocyte was used to stimulate the nerve stem cells

and H89

PKA recepter antagonist

was used to pretreat the nerve stem cells of the cerebral cortex

then the contents of PKA

nestin

and NSF in the nerve stem cells were determined using western blot. MTT was applied to determine and analyse the effect of horizontal cell on the proliferation and orientational dif- ferentiation of cultured nerve stem cells. Results:In the normal control group the content of NSF in the nerve stem cells was low;after astrocyte stimulation the nestin content decreased rapidly

while the content of NSF increased gradually. H8(915 μmol/L) could markedly supress the increase of NSF content in the nerve stem cell stimulated by horizontal cells. Conclusion:Astrocyte can promote the proliferation and differentiation of nerve stem cells by activating PKA signal pathway of nerve stem cells.