Preliminary study effect of artificial liver support on HBV cccDNA in patients with fulminant hepatitis B

YANG Pei; et al

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Preliminary study effect of artificial liver support on HBV cccDNA in patients with fulminant hepatitis B

更新时间：2024-01-17

- Preliminary study effect of artificial liver support on HBV cccDNA in patients with fulminant hepatitis B
- Journal of Chongqing Medical University Vol. 33, Issue 12, Pages: 1463-1466(2008)
- 作者机构：
  
  1. 重庆市血液中心成份科
  2. 重庆医科大学附属第二医院感染病科
  3. 重庆医科大学病毒性肝炎研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC： R512.62
- Published：2008，
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[1]杨培,秦波,单幼兰,刘杞.人工肝支持系统对重型乙型肝炎患者血清中HBV cccDNA影响的初步研究[J].重庆医科大学学报,2008,33(12):1463-1466.

YANG Pei, et al. Preliminary study effect of artificial liver support on HBV cccDNA in patients with fulminant hepatitis B. [J]. Journal of Chongqing Medical University 33(12):1463-1466(2008)
[1]杨培,秦波,单幼兰,刘杞.人工肝支持系统对重型乙型肝炎患者血清中HBV cccDNA影响的初步研究[J].重庆医科大学学报,2008,33(12):1463-1466. DOI：

YANG Pei, et al. Preliminary study effect of artificial liver support on HBV cccDNA in patients with fulminant hepatitis B. [J]. Journal of Chongqing Medical University 33(12):1463-1466(2008) DOI：

摘要

目的:建立HBV cccDNA荧光定量PCR方法并检测重型乙型肝炎患者人工肝血浆置换治疗前后血清中HBVcccDNA含量。初步探索人工肝支持系统对重型乙型肝炎患者血清中HBV cccDNA的影响。方法:以50例重型乙型肝炎患者人工肝血浆置换治疗前后血清作为检测标本

对血清中总HBV DNA、HBV cccDNA含量进行定量检测。用SAS8.0统计软件对结果进行统计学分析。结果:成功建立定量检测HBV cccDNA方法并证实在重型乙型肝炎患者血清中存在HBV cccDNA。在人工肝血浆置换前后总HBV DNA及HBV cccDNA含量明显降低（P<0.05）。总HBV DNA与HBV cccDNA之间有较好的相关性（r=0.78

P<0.01）。总HBV DNA水平与PT（r=0.37

P<0.01）、ALT（r=0.29

P<0.05）、AST（r=0.39

P<0.01）水平有一定的相关性;HBV cccDNA水平与PT（r=0.34

P<0.05）、ALT（r=0.34

P<0.05）、AST（r=0.40

P<0.01）水平有一定的相关性。结论:总HBV DNA及HBV cccDNA在一定程度上能够反映肝细胞坏死程度;血浆置换术后病毒含量明显降低

为进一步研究重型乙型肝炎治疗途径及发病机制打下基础。

Abstract

Objective:To establish a new fluorescence-based quantitative PCR assay for detecting hepatitis B virus covalently closed circular DNA(HBV cccDNA).To explore the effect of artificial liver support system(ALSS)on total HBV DNA and HBV cccDNA in sera of patients with fulminant hepatitis B.Methods:The serum specimens from 50 patients of fulminant hepatitis B prior to and after treatment with ALSS were collected.Then HBV cccDNA was quantitatively detected by fluorescent PCR with specific primer set and Taqman probe.And the results were analyzed by SAS8.0 statistics software.Results:We successfully established a new fluorescence-based quantitative PCR method for HBV cccDNA.HBV cccDNA was detectable in sera of the patients with fulminant hepatitis B.The level of total HBV DNA and HBV cccDNA decreased significantly following plasma exchange in ALSS.The levels of total HBV DNA and HBV cccDNA had positive correlation with degree of PT

ALT

andAST.Total HBV DNA also had positive correlation with HBV cccDNA.Conclusion:The level of total HBV DNA and HBV cccDNA has positive correlation with degree of PT

ALT

and AST

which might be indication of hepatocyte damage.The finding that significant decrease in total HBV DNA and HBV cccDNA after plasma exchange in ALSS may provide a clue of further research about the fulminant hepatitis B treated with ALSS.

关键词

肝炎病毒乙型共价闭合环状DNA荧光定量PCR重型乙型肝炎人工肝支持系统

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A method for detecting intracellular HBV covalently closed circular DNA in cultured cells in vitro

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Key Laboratory of Molecular Biology on Infectious Diseases，Ministry of Education， Chongqing Medical University

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