Neuroprotective of neuroglobin induced by hemin in the hippocampal organotypic tissue cultures after combined oxygen/glucose deprivation

WANG Li; et al

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Neuroprotective of neuroglobin induced by hemin in the hippocampal organotypic tissue cultures after combined oxygen/glucose deprivation

更新时间：2024-01-17

- Neuroprotective of neuroglobin induced by hemin in the hippocampal organotypic tissue cultures after combined oxygen/glucose deprivation
- Journal of Chongqing Medical University Vol. 34, Issue 2, Pages: 138-141(2009)
- 作者机构：
  
  1. 重庆市肿瘤研究所肿瘤科
  2. 重庆医科大学附属第一医院神经内科
  3. 第三军医大学大坪医院野战外科研究所病理科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R741
- Published：2009，
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[1]王莉,余刚,向德兵,朱洁,李光勤.海马脑片氧糖剥离模型中血晶素对脑红蛋白表达的影响[J].重庆医科大学学报,2009,34(02):138-141.

WANG Li, et al. Neuroprotective of neuroglobin induced by hemin in the hippocampal organotypic tissue cultures after combined oxygen/glucose deprivation. [J]. Journal of Chongqing Medical University 34(2):138-141(2009)
[1]王莉,余刚,向德兵,朱洁,李光勤.海马脑片氧糖剥离模型中血晶素对脑红蛋白表达的影响[J].重庆医科大学学报,2009,34(02):138-141. DOI：

WANG Li, et al. Neuroprotective of neuroglobin induced by hemin in the hippocampal organotypic tissue cultures after combined oxygen/glucose deprivation. [J]. Journal of Chongqing Medical University 34(2):138-141(2009) DOI：

摘要

目的:观察血晶素（Hemin）对海马脑片氧糖剥离（Oxygen/glucose deprivation

OGD）模型的保护作用及其对脑红蛋白（Neuroglobin

Ngb）表达的影响。方法:取新生8

0dSD大鼠

制备厚约400μm海马脑片

培养2周后筛取完好无特异荧光着色的脑片

随机分为3组:正常培养对照组（HOTC）

单纯缺氧缺糖组（OGD）

预加Hemin再缺氧缺糖组（Hemin+OGD）。除HOTC组外

其余各组在5%CO2、95%N2的混合气体中培养1.5h

其后恢复培养24h。冰冻连续冠状切片

分别作尼氏染色、Ngb的免疫组织化学染色。应用免疫组织化学染色和图像分析处理技术观察海马脑片Ngb蛋白表达

并观察神经元形态学改变。结果:OGD组锥体细胞发生坏死

锥体细胞数量明显减少;Hemin+OGD组坏死较前者轻

锥体细胞数较前者多。OGD组海马脑片中形态结构正常的阳性细胞少

多数阳性神经元结构模糊、肿胀

Ngb的表达强于HOTC组

二者相比有显著性差异（P

0.05）;Hemin+OGD组海马脑片中大部分细胞形态结构正常

少数阳性神经元结构模糊、肿胀

Ngb蛋白的表达较HOTC组和OGD组明显增强（P

0.01）。结论:血晶素可减轻海马脑片缺糖缺氧性损伤

其机制可能与其诱导神经元表达Ngb密切相关。

Abstract

Objective:To investigate the neuroprotective role of hemin and its effect on the expression of neuroglobin in the hippocampal organotypic tissue cultures(HOTC)after combined oxygen/glucose deprivation(OGD).Methods:SD rats

8~10-days old

were sacri-ficed for brain.HOTC were made and randomly divided into three groups:HOTC

HOTC undergoing OGD(OGD)

HOTC under- going OGD following pretreatment with hemin(Hemin+OGD). The expression of neuroglobin was detected by immunohisto-chemical technique and computer-assisted image analysis.Results:In OGD group

hippocampal organotypic tissue lost normal structure and neurons were damaged severely.Pretreatment with hemin could effectively reverse the damage of the neurons.The expression of neu- roglobin in the Hemin+OGD group were significantly stronger than that in the OGD group.Conclusion:Hemin has protective effect on injury induced by OGD in rat hippaocampal slices

which is associated with enhanced expression of neuroglobin.

关键词

海马脑片缺氧缺糖脑红蛋白血晶素

Keywords

Hippocampal organotypic tissue culture(sHOTC)Oxygenl/glucose deprivation(OGD)neuroglobinHemin

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