Evaluation synergism of GLS/IL-12 recombinant M.s megmatis to anti-tuberculosis chemotherapy in murine model of M.tuberculosis infection

YANG Xiao-yan; et al

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Evaluation synergism of GLS/IL-12 recombinant M.s megmatis to anti-tuberculosis chemotherapy in murine model of M.tuberculosis infection

更新时间：2024-01-17

- Evaluation synergism of GLS/IL-12 recombinant M.s megmatis to anti-tuberculosis chemotherapy in murine model of M.tuberculosis infection
- Journal of Chongqing Medical University Vol. 34, Issue 5, Pages: 516-520(2009)
- 作者机构：
  
  1. 重庆医科大学基础医学院免疫学教研室
  2. 重庆医科大学基础医学院神经科学研究中心
  3. 重庆医科大学基础医学院病原生物学教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R52
- Published：2009，
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[1]杨晓燕,杨春,何永林,徐蕾.重组耻垢分枝杆菌联合抗痨药物对鼠结核病治疗作用的探讨[J].重庆医科大学学报,2009,34(05):516-520.

YANG Xiao-yan, et al. Evaluation synergism of GLS/IL-12 recombinant M.s megmatis to anti-tuberculosis chemotherapy in murine model of M.tuberculosis infection. [J]. Journal of Chongqing Medical University 34(5):516-520(2009)
[1]杨晓燕,杨春,何永林,徐蕾.重组耻垢分枝杆菌联合抗痨药物对鼠结核病治疗作用的探讨[J].重庆医科大学学报,2009,34(05):516-520. DOI：

YANG Xiao-yan, et al. Evaluation synergism of GLS/IL-12 recombinant M.s megmatis to anti-tuberculosis chemotherapy in murine model of M.tuberculosis infection. [J]. Journal of Chongqing Medical University 34(5):516-520(2009) DOI：

摘要

目的:探讨携带编码人天然颗粒溶素（Granulysin

GLS）和小鼠IL-12基因的重组耻垢分枝杆菌（M.smegmatis

MS）与抗痨药物对结核分枝杆菌感染的协同治疗效果。方法:将结核分枝杆菌H37Rv感染的Balb/c小鼠40只随机分成4组。感染4周后分别用生理盐水、重组MS、INH+PZA和重组MS联合INH+PZA治疗

于第一次治疗后3个月

处死小鼠

检测器官荷菌量、脾淋巴细胞IFN-γ和TNF-α的分泌水平、血清IL-12和IFN-γ和肺、脾组织中GLS表达

同时观察小鼠肺、脾组织病理改变情况。结果:重组MS联合INH+PZA治疗组肺、脾组织荷菌量（lgCFU/g）比重组MS和INH+PZA治疗组显著降低;重组MS和INH+PZA治疗组肺、脾组织荷菌量比生理盐水组显著降低。重组MS组经PPD刺激后IFN-γ和TNF-α分泌水平明显高于其它组。重组MS联合INH+PZA治疗组和重组MS组血清IL-12和IFN-γ水平明显高于生理盐水组。重组MS和INH+PZA组病变轻且局限

生理盐水组肺组织病理改变以渗出为主

病变广泛。重组MS联合INH+PZA治疗组肺组织病变最轻。结论:GLS/IL-12重组MS对小鼠结核病有一定免疫治疗作用;重组MS联合临床常用的抗痨药可增强抗结核病的疗效。

Abstract

Objective:To evaluate the synergism ofrecombinant M.smegmatis(MS)encoding human granulysin and murine IL-12 to anti-tuberculosis chemotherapy in murine M.tuberculosis M.tuberculosis infection.Methods:Balb/c mice were infected with normal saline

recombinant MS

INH(Isoniazid)+PZA(pyrazinamide)

recombinant MS+INH+PZA respectively.The numbers of viable bacteria in the lung and the spleen were counted.The levels of IL12 and IFN-γin serum and IFN-γand TNF-αreleased from spleen lymphocytes stimulated with PPD were detected with ELISA.Lungs and spleens were prepared for pathological analysis.Results:The recombinant MS group

INH+PZA group

and recombinant MS+INH+PZA group showed significantly reduced number of colony forming units(log CFU/g) compared with saline group.The increase of IL-12 and IFN-γin serum were found in recombinant MS group.IFN-γand TNF-αreleased from spleen lymphocytes stimulated with PPD in recombinant MS group was higher than that in other groups(P<0.01).The pathological changes in lungs of the recombinant MS group

INH(Isoniazid)+PZA(pyrazinamide)group

and recombinant MS+INH+PZA group were localized

while those in the control group were extensive.The significant pathological changes were not found in the spleens of all groups.Conclusion:The recombinant MS had immunotherapeutic effects

the recombinant MS had a synergism to anti-tuberculosis chemotherapy.

关键词

颗粒溶素IL-12重组MS结核病抗痨药

Keywords

GranulysinIL-12Recombinant MStuberculosisAnti-tuberculosis chemotherapy

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