Mechanism of apoptosis in hippocampus of the rats with disorder of cortical developments

HUANG Hua; et al

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Mechanism of apoptosis in hippocampus of the rats with disorder of cortical developments

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- Mechanism of apoptosis in hippocampus of the rats with disorder of cortical developments
- Journal of Chongqing Medical University Vol. 34, Issue 2, Pages: 129-134(2009)
- 作者机构：
  
  重庆医科大学附属第一医院神经内科重庆市神经病学重点实验室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R742.1
- Published：2009，
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[1]黄华,冯占辉,何选丽,黄敏,晏勇.皮质发育障碍模型鼠海马区神经元凋亡机制的研究[J].重庆医科大学学报,2009,34(02):129-134.

HUANG Hua, et al. Mechanism of apoptosis in hippocampus of the rats with disorder of cortical developments. [J]. Journal of Chongqing Medical University 34(2):129-134(2009)
[1]黄华,冯占辉,何选丽,黄敏,晏勇.皮质发育障碍模型鼠海马区神经元凋亡机制的研究[J].重庆医科大学学报,2009,34(02):129-134. DOI：

HUANG Hua, et al. Mechanism of apoptosis in hippocampus of the rats with disorder of cortical developments. [J]. Journal of Chongqing Medical University 34(2):129-134(2009) DOI：

摘要

目的:探讨皮质发育障碍（Disorder of cortical developments

DCDs）模型鼠海马神经元的凋亡机制。方法:利用卡莫司汀（BCNU）诱导建立SD大鼠皮质发育障碍动物模型

在出生后0d（P0）、15d（P15）、30d（P30）、45d（P45）和60d（P60）时

采用TUNEL检测海马区神经元凋亡

RT-PCR方法和免疫组化分别对8只模型鼠和正常鼠检测海马区Bcl-2和Bax的在mRNA和蛋白水平的表达

并结合图像分析系统进行结果分析。结果:成功建立SD大鼠皮质发育障碍动物模型;与对照组比较

TUNEL、免疫组化和RT-PCR检测结果显示:P0模型鼠组海马区神经元凋亡无明显变化

P15、P30、P45、P60模型组神经元凋亡逐渐加重

有显著差异（P<0.05）

且随存活时间延长

模型组海马神经元Bax表达逐渐增强

Bax/Bcl-2比值较对照组增高

具有显著统计学意义（P<0.05）。结论:皮质发育障碍模型鼠海马区存在显著的神经元凋亡

Bcl-2和Bax参与了其凋亡过程;皮质发育障碍模型鼠海马神经元凋亡可能参与了皮质发育障碍所致的癫痫及难治性癫痫的发病机制。

Abstract

Objective:To study the mechanism of apoptosis in hippocampus of the rats with disorder of cortical developments.Methods:The Rats with disorder of cortical developments were established by carmustine(BUCN).AT P0

P15

P30

P45

P60

the number of apoptosis cell and expression of Bax and Bcl-2 in hippocampus were detected by TUNEL

immunohistochemistry method and RT-PCR;then the detected outcomes were analyzed by computer micrograph analysis system in control group and model group.Results:At P15

P30

P45

P60

the numbers of apoptosis

Bax

Bcl-2 masculine cell and bcl-2 MRNA

bax MRNA in hippocampus in the model group were more than those in the control group.The ratio of Bax/Bcl-2 in model group was significantly higher than those in the control group(P<0.05)

but at P0

they were not different.Conclusion:Apoptosis exist notably in hippocampus of rat with disorder of cortical develop-ments.bcl-2 and bax probably play a role in apoptosis.Apoptosis may be associated with the pathogenesis of epilepsy or intractable epilepsy.

关键词

皮质发育障碍细胞凋亡BaxBcl-2癫痫

Keywords

Disorder of cortical developmentscell apoptosisBaxBcl-2epilepsy

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