Relationship of gene expression and activity of chymase and Ang Ⅱ,nephropathy in streptozotocin-induced diabetic hamsters

CHENG Li; et al

您当前的位置：

首页 >

文章列表页 >

Relationship of gene expression and activity of chymase and Ang Ⅱ,nephropathy in streptozotocin-induced diabetic hamsters

更新时间：2024-01-17

- Relationship of gene expression and activity of chymase and Ang Ⅱ,nephropathy in streptozotocin-induced diabetic hamsters
- Journal of Chongqing Medical University Vol. 34, Issue 8, Pages: 1011-1014(2009)
- 作者机构：
  
  1. 重庆医科大学附属第一医院老年病科
  2. 四川省成都市金牛区成都铁路中心医院内分泌科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R587.2
- Published：2009，
扫描看全文
[1]程黎,肖谦,蒲霞,李龙英,赵柯湘,高原.糖尿病仓鼠肾脏chymase mRNA表达及酶活性与AngⅡ和肾脏病变关系的实验研究[J].重庆医科大学学报,2009,34(08):1011-1014.

CHENG Li, et al. Relationship of gene expression and activity of chymase and Ang Ⅱ,nephropathy in streptozotocin-induced diabetic hamsters. [J]. Journal of Chongqing Medical University 34(8):1011-1014(2009)
[1]程黎,肖谦,蒲霞,李龙英,赵柯湘,高原.糖尿病仓鼠肾脏chymase mRNA表达及酶活性与AngⅡ和肾脏病变关系的实验研究[J].重庆医科大学学报,2009,34(08):1011-1014. DOI：

CHENG Li, et al. Relationship of gene expression and activity of chymase and Ang Ⅱ,nephropathy in streptozotocin-induced diabetic hamsters. [J]. Journal of Chongqing Medical University 34(8):1011-1014(2009) DOI：

摘要

目的:探讨糖尿病仓鼠肾脏chymase mRNA表达及酶活性与血管紧张素Ⅱ（AngiotensinⅡ

AngⅡ）水平和肾脏病变的关系。方法:30只8周龄雄性仓鼠随机分为正常对照组（n=10）和糖尿病组（n=20）

腹腔内注射链脲菌素制备糖尿病仓鼠模型

电镜观察肾脏超微结构

反转录-聚合酶链反应（RT-PCR）检测chymase mRNA水平

放射免疫分析法测定肾脏chymase、血管紧张素转换酶（Angiotensin-convertion enzyme

ACE）活性和AngⅡ含量。结果:（1）糖尿病组24h尿白蛋白含量高于对照组

（2.32±0.18）mgvs（0.11±0.01）mg

P<0.01

透射电镜发现糖尿病组肾小球毛细血管基底膜增厚

系膜细胞间细胞外基质沉积;（2）与对照组相比

糖尿病组肾脏chymasemRNA表达水平明显增高（4.90±0.79vs1.81±0.09

P<0.01）

酶活性也明显增高

（0.82±0.05）Uvs（0.49±0.03）U

P<0.01

AngⅡ含量明显增高

（88.6±17.5）pg/mgvs（48.2±15.3）pg/mg

P<0.01

ACE活性无差异（P>0.05）。结论:糖尿病仓鼠肾脏chymase mRNA表达水平和酶活性明显增高可能是AngⅡ水平升高的主要原因

chymase可能有关于糖尿病肾病（Diabetic nephropathy

DN）的发生。

Abstract

Objective:To investigate the relationship of gene expression and activity of chymase and Ang Ⅱwith nephropathy in the streptozotocin(STZ)-induced diabetic hamsters.Methods:Thirty male hamsters were randomly divided into two groups:normal controls and diabetic rats.Diabetes mellitus was induced in rats by intraperitoneal streptozotocin injection.The nephritic ultrastructure was observed with electron microscope.The levels of blood glucose

lipoprotein were measured by biochemical methods.The level of angiotensin Ⅱ(Ang Ⅱ) and the activity of chymase and angiotensin-convertion enzyme(ACE) were measured by radioimmuno assay.Reverse transcription polymerase chain reaction(RT-PCR)was used to determine chymase gene expression(corrected by β-actin).Results:Chymase activity in DN group was much higher than that in the control group(0.82±0.05) U vs(0.49±0.03) U

P<0.01.This was accompanied by increased angiotensin Ⅱ(88.6±17.5) pg/mg tissue vs(48.2±15.3) pg/mg tissue

P<0.01 and chymase mRNA(1.81±0.09) vs(4.90±0.79)

P<0.01.But the activity of ACE was unchanged

indicating an alternative non-ACE dependent way of angiotensin Ⅱgenerating.Conclusion:In diabetic hamsters

the gene expression and activity of chymase are much higher than those in the control group

accompanying higher level of angiotensin Ⅱ.This reflects another mechanism was involved except for the classic ACE pathway to activate the intrarenal RAS in the progress of diabetic nephropathy(DN).

关键词

Chymase糖尿病肾病血管紧张素Ⅱ

Keywords

Chymasediabetic nephropathyAngiotensin Ⅱ

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Experimental study on angiotensin Ⅱ and podocyte injury in diabetic rats

Carvacrol alleviates oxidative stress damage in rats with diabetic nephropathy via the SIRT1/FOXO1 pathway

Protection of sodium glucose cotransporter-2 inhibitors on diabetic nephropathy

Effects of HIF1α-KIM1 signaling pathway on renal interstitial fibrosis in diabetic nephropathy

URINARY N-ACETY1-β-D-GLUCOSAMIMDASE DETERMINATION FOR DETECTION OF DIABETIC NEPHROPATHY AT EARLYSTAGE

Related Author

刘东方

兰丽珍

曹文富

et al

QIN Qiaojing

Liu Xia

Yang Bingqi

Related Institution

Department of Endocrinology,College of Clinical Medicine,Chongqing Medical University

重庆医科大学临床学院内分泌科

Department of Obstetrics and Gynecology，Hebei Provincial Hospital of Traditional Chinese Medicine

河北省中医院导管室

Department of Endocrinology,The First Affiliated Hospital of Chongqing Medical University

⁰