Effects of valproate sodium on phosphorylatted cAMP responsive element binding protein after hippocampal neuronal epileptiform discharge in rat

LIU Hua; et al

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Effects of valproate sodium on phosphorylatted cAMP responsive element binding protein after hippocampal neuronal epileptiform discharge in rat

更新时间：2024-01-17

- Effects of valproate sodium on phosphorylatted cAMP responsive element binding protein after hippocampal neuronal epileptiform discharge in rat
- Journal of Chongqing Medical University Vol. 34, Issue 8, Pages: 1024-1027(2009)
- 作者机构：
  
  1. 重庆市第三人民医院神内科
  2. 重庆医科大学附属第二医院神经内科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R742.1
- Published：2009，
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[1]刘华,徐祖才,陈阳美.丙戊酸钠对大鼠海马神经元癫痫样放电后P-CREB1表达的影响[J].重庆医科大学学报,2009,34(08):1024-1027.

LIU Hua, et al. Effects of valproate sodium on phosphorylatted cAMP responsive element binding protein after hippocampal neuronal epileptiform discharge in rat. [J]. Journal of Chongqing Medical University 34(8):1024-1027(2009)
[1]刘华,徐祖才,陈阳美.丙戊酸钠对大鼠海马神经元癫痫样放电后P-CREB1表达的影响[J].重庆医科大学学报,2009,34(08):1024-1027. DOI：

LIU Hua, et al. Effects of valproate sodium on phosphorylatted cAMP responsive element binding protein after hippocampal neuronal epileptiform discharge in rat. [J]. Journal of Chongqing Medical University 34(8):1024-1027(2009) DOI：

摘要

目的:研究丙戊酸钠对大鼠海马神经元癫痫样放电后磷酸化腺感苷反应元件结合蛋白1（Phosphorylated cAMP responsive element binding protein 1

P-CREB1）表达的影响。方法:wistar新生鼠

迅速断头取脑

体外培养海马神经元

建立神经元癫痫样放电模型

将神经元分为空白组、模型组、丙戊酸钠低剂量（50mg/L）组、丙戊酸钠高剂量（100mg/L）组

运用免疫荧光技术观察P-CREB1在神经元癫痫样放电后在细胞内的表达部位

采用wester blot技术测定P-CREB1在不同分组中的表达强度。结果:通过免疫荧光技术

在各组中都可以看到P-CREB1在细胞核内表达

以模型组最明显;运用Western blot

发现表达趋势与免疫荧光一致

并且

给予丙戊酸钠后

P-CREB1表达减弱

且高剂量组与低剂量组之间差异有统计学意义（P<0.05）。结论:海马神经元无镁处理后呈癫痫样放电

同时P-CREB1被过度激活

而有效浓度的丙戊酸钠可抑制此反应P-CREB1的磷酸化水平。

Abstract

Objective:To investigate the effects of valproate sodium on P-CREB1 after hippocampal neuronal epileptiform discharge in rat.Methods:The neonate wistar rats were decapitated quickly to obtain the hippocampal neuron

Which were cultured in vitro

After the epileptiform discharge model of neuron was established

neurons were divided into control group

model group

low valproate dose group(50mg/L) and high dose group(100mg/L).Expression positions of P-CREB1 after epileptiform discharge were examined by immunofluorescence technique

and Western blot was used to examine the expression intensity of P-CREB1 in different group.Results:Through immunofluorescence

P-CREB1 was observed in the nucleus of each group

and the most intensive expression was found in model group.Through western blot

the expression tendency was found to be the same as the former Results

Moreover

after added valproate sodium

the expression of P-CREB1 decreased

and there was statistical significance of the difference between low valproate dose and high dose(P<0.05).Conclusion:P-CREB1 could be persistently activated in the hippocampal neuron

which presents epileptiform discharge.Valproate sodium could decrease the phosphorylation of P-CREB1 in effective dose.

关键词

丙戊酸钠P-CREB1神经元癫痫样放电

Keywords

Valproate sodiumP-CREB1neuronEpileptiform discharge

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Department of Neurology,the Children’s Hospital,Chongqing Medical University

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