Effect of silencing annexin Ⅱ by specific siRNA on invasion and metastasis of human breast cancer cell MDA-MB-231

GUO Bian-qin; et al

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Effect of silencing annexin Ⅱ by specific siRNA on invasion and metastasis of human breast cancer cell MDA-MB-231

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- Effect of silencing annexin Ⅱ by specific siRNA on invasion and metastasis of human breast cancer cell MDA-MB-231
- Journal of Chongqing Medical University Vol. 34, Issue 8, Pages: 984-987(2009)
- 作者机构：
  
  1. 重庆医科大学医学检验系血液学教研室、教育部重点实验室、重庆市重点实验室
  2. 重庆医科大学附属第一医院检验科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.9
- Published：2009，
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[1]郭变琴,文阳安,陈婷梅.特异性小干扰RNA沉默Annexin Ⅱ基因表达对人乳腺癌细胞MDA-MB-231侵袭迁移力的影响[J].重庆医科大学学报,2009,34(08):984-987.

GUO Bian-qin, et al. Effect of silencing annexin Ⅱ by specific siRNA on invasion and metastasis of human breast cancer cell MDA-MB-231. [J]. Journal of Chongqing Medical University 34(8):984-987(2009)
[1]郭变琴,文阳安,陈婷梅.特异性小干扰RNA沉默Annexin Ⅱ基因表达对人乳腺癌细胞MDA-MB-231侵袭迁移力的影响[J].重庆医科大学学报,2009,34(08):984-987. DOI：

GUO Bian-qin, et al. Effect of silencing annexin Ⅱ by specific siRNA on invasion and metastasis of human breast cancer cell MDA-MB-231. [J]. Journal of Chongqing Medical University 34(8):984-987(2009) DOI：

摘要

目的:探讨RNA干扰技术沉默Annexin Ⅱ基因表达对人乳腺癌细胞MDA-MB-231侵袭迁移力的影响。方法:体外化学合成Annexin Ⅱ基因序列特异性双链小干扰RNA（Annexin Ⅱ-siRNA）

转染高转移人乳腺癌细胞株MDA-MB-231

用荧光显微镜观察转染效率

逆转录-聚合酶链反应和蛋白印迹检测Annexin Ⅱ mRNA和蛋白表达水平;通过生长曲线和体外侵袭迁移实验

观察乳腺癌细胞恶性生物学行为的改变。结果:Annexin Ⅱ-siRNA能有效抑制Annexin Ⅱ mRNA和蛋白表达（P<0.05）;经siRNA处理的细胞

细胞生长速度明显降低（P<0.05）

侵袭迁移力显著下降（P<0.05）。结论:沉默Annexin Ⅱ基因表达可抑制乳腺癌细胞MDA-MB-231的侵袭迁移力。

Abstract

Objectives:To investigate the effects of small interference RNA(siRNA) silencing Annexin Ⅱ on invasion and metastasis of human breast cancer cell MDA-MB-231.Methods:The chemically-synthetic siRNA duplexes targeting Annexin Ⅱ(Annexin Ⅱ-siRNA) was transiently transfected into MDA-MB-231 cells which have high metastatic potential by lipofectamin 2000.The transfection efficiency was observed by fluorescence microscopy.RT-PCR and western blot were used to semi-quantify the Annexin Ⅱ mRNA and protein levels.The malignant characters of transfected MDA-MB-231 cells including celluar proliferation rate

the activities of invasion and metastasis were analyzed by use of 3-(4

5-dimethylthiazol-2-yl)-5-diphenyl tetrazolium bromide(MTT) and millicell chamber assay

respectivilly.Results:Annexin Ⅱ-siRNA effectively inhibited Annexin Ⅱ mRNA and protein levels(P<0.05).With siRNA interferring cells

the ability of cells proliferation was decreased(P<0.05)

and cells invasive and metastasis were depressed(P<0.05).Conclusion:Silencing Annexin Ⅱ could significantly inhibit the abilities of invasion and metastasis of human breast cancer cells MDA-MB-231 in vitro.

关键词

RNA干扰乳腺癌Annexin Ⅱ侵袭转移

Keywords

RNA interferencebreast cancerAnnexinⅡinvasion and metastasis

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