Construction and identification of plasmid vector of short interfering RNA on pneumocystis carinii thymidylate synthase gene

TANG Ying-hui; et al

您当前的位置：

首页 >

文章列表页 >

Construction and identification of plasmid vector of short interfering RNA on pneumocystis carinii thymidylate synthase gene

更新时间：2024-01-17

- Construction and identification of plasmid vector of short interfering RNA on pneumocystis carinii thymidylate synthase gene
- Journal of Chongqing Medical University Vol. 34, Issue 7, Pages: 877-879(2009)
- 作者机构：
  
  重庆医科大学附属第一医院感染科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R346;R531.5
- Published：2009，
扫描看全文
[1]唐滢浍,姚云清,谭燕,王莉妮,李文桂,刘成伟,陈雅棠.卡氏肺孢子胸腺嘧啶核苷酸合酶基因siRNA表达载体的构建和鉴定[J].重庆医科大学学报,2009,34(07):877-879.

TANG Ying-hui, et al. Construction and identification of plasmid vector of short interfering RNA on pneumocystis carinii thymidylate synthase gene. [J]. Journal of Chongqing Medical University 34(7):877-879(2009)
[1]唐滢浍,姚云清,谭燕,王莉妮,李文桂,刘成伟,陈雅棠.卡氏肺孢子胸腺嘧啶核苷酸合酶基因siRNA表达载体的构建和鉴定[J].重庆医科大学学报,2009,34(07):877-879. DOI：

TANG Ying-hui, et al. Construction and identification of plasmid vector of short interfering RNA on pneumocystis carinii thymidylate synthase gene. [J]. Journal of Chongqing Medical University 34(7):877-879(2009) DOI：

摘要

目的:构建和鉴定大鼠卡氏肺孢子（Pneumocystis carinii

PC）胸腺嘧啶核苷酸合酶（Thymidylate synthase

TS）基因的siRNA（Short interfering RNA）表达载体。方法:人工合成针对PCTS基因的1对shRNA（Short hairpin RNA）序列并定向克隆到空载体pTZU6+1上

构建siRNA表达重组质粒pPC-TS

并采用双酶切产物凝胶电泳和基因测序法鉴定。结果:酶切电泳和基因测序鉴定得到的产物与预期的目的基因一致。结论:成功构建卡氏肺孢子胸腺嘧啶核苷酸合酶基因siRNA表达载体。

Abstract

Objective:To construct and identify a plasmid vector of short interfering RNA(siRNA) on pneumocystis carinii (PC) thymidylate synthase. Methods: Short hairpin RNA oligonucleotides of thymidylate synthase were chemically synthesized and inserted into plasmid vector pTZU6+1 after annealing. The recombinant plasmid

pPC-TS

transformed into E. coil. TOP10 and amplified

was digested by restriction endonucleases Hind Ⅲ and EcoR Ⅰ and identified by gel electrophoresis and DNA sequencing. Results: Gel electrophoresis and DNA sequencing showed that the recombinant plasmid containing the correct and full shRNA oligonucleotide. Conclusion:The siRNA plasmid

pPC-TS

was constructed successfully.

关键词

卡氏肺孢子胸腺嘧啶核苷酸合酶基因siRNA表达载体

Keywords

Pneumocystis cariniiThymidylate synthase genesiRNA expression plasmid

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Construction and identification of plasmid vector of short interfering RNA on pneumocystis carinii major surface gene

Related Author

WANG Li-ni

姚云清

谭燕

唐滢浍

李文桂

马良

陈雅堂

Related Institution

重庆医科大学附属第一医院感染科

⁰