Influence of dendritic cells modified with ICOS extracellular region on survival of renal allografts in rats

FAN Ming-qi; et al

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Influence of dendritic cells modified with ICOS extracellular region on survival of renal allografts in rats

更新时间：2024-01-17

- Influence of dendritic cells modified with ICOS extracellular region on survival of renal allografts in rats
- Journal of Chongqing Medical University Vol. 34, Issue 9, Pages: 1191-1192(2009)
- 作者机构：
  
  第三军医大学附属新桥医院泌尿外二科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R699.2
- Published：2009，
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[1]范明齐,冯嘉瑜,肖亚,黄赤兵.ICOS胞外区基因修饰的树突状细胞对移植肾存活的影响[J].重庆医科大学学报,2009,34(09):1191-1192.

FAN Ming-qi, et al. Influence of dendritic cells modified with ICOS extracellular region on survival of renal allografts in rats. [J]. Journal of Chongqing Medical University 34(9):1191-1192(2009)
[1]范明齐,冯嘉瑜,肖亚,黄赤兵.ICOS胞外区基因修饰的树突状细胞对移植肾存活的影响[J].重庆医科大学学报,2009,34(09):1191-1192. DOI：

FAN Ming-qi, et al. Influence of dendritic cells modified with ICOS extracellular region on survival of renal allografts in rats. [J]. Journal of Chongqing Medical University 34(9):1191-1192(2009) DOI：

摘要

目的:探讨诱导共刺激分子（Inducible costimulator

ICOS）胞外区基因修饰的树突状细胞（Dendriti ccells

DC）对移植肾存活的影响。方法:以BN、Lewis大鼠为肾移植供、受体;用ICOS胞外区基因重组腺病毒转染供体骨髓源性DC使之表达ICOS胞外区;将供体DC于肾移植前24h经股静脉注入受体为实验组

未注射DC的受体为对照组;观察两组移植肾存活时间

术后第20d

MTT法检测受体脾细胞对供体及无关抗原刺激的反应。结果:与对照组相比

实验组移植肾存活时间显著延长（[23.2±3.08）d与（8.5±1.4）d]

P<0.01)。术后第20d

实验组脾细胞对供体抗原刺激的反应明显低于正常对照（0.37±0.06）与（0.84±0.12）

P<0.01

而对无关抗原刺激的反应与正常对照差异无显著性（0.76±0.11）与（0.69±0.09）

P>0.05。结论:I-COS胞外区基因修饰的供体DC可以明显延长大鼠移植肾存活时间。

Abstract

Objective:To study the influence of donor dendritic cells (DCs) modified with ICOS extracellular region on the survival of renal allografts in rats. Methods:Bone marrow derived DCs of Brown Norway(BN) rats were modified with ICOS extracellular region gene recombined adenovirus and injected into Lewis rats 24 h before BN→Lewis kidney transplantation. Survival time of renal allografts was observed and one-way mixed splenic cell reaction (MSR) of the recipients to donors and the third party rats were performed by means of MTT on the 20th postoperative day. Results:Survival time of renal allografts was prolonged significantly [comparedwith control

(23.2±3.08) d vs(8.5±1.4) d

P <0.01]. On the 20th day

the recipients showed significant hyporesponsiveness to donors (compared with normal control

MSR was(0.37±0.06) vs(0.84±0.12)

P<0.01

but showed normal reaction to the third party.Conclusion:Donor DCs modified with ICOS extracellular region can induce a significant prolongation of renal allografts survival.

关键词

ICOS树突状细胞肾移植

Keywords

ICOSdendritic cellskidney transplantation

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