The expression of Inhibitor of apoptosis protein of Livin and Survivin induced by 5-Fu in hepatocelluar carcinoma(HCC)

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The expression of Inhibitor of apoptosis protein of Livin and Survivin induced by 5-Fu in hepatocelluar carcinoma(HCC)

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- The expression of Inhibitor of apoptosis protein of Livin and Survivin induced by 5-Fu in hepatocelluar carcinoma(HCC)
- Journal of Chongqing Medical University Vol. 34, Issue 7, Pages: 849-851(2009)
- 作者机构：
  
  1. 重庆医科大学附属第一医院肝胆外科
  2. 重庆医科大学附属第一医院外科实验室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R735.7
- Published：2009，
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[1]吴桂新,王洪林,邓开,郑科,董蒲江.Livin,Survivin在肝癌细胞的表达及5-Fu对其的诱导作用[J].重庆医科大学学报,2009,34(07):849-851.

WU Gui-xin, et al. The expression of Inhibitor of apoptosis protein of Livin and Survivin induced by 5-Fu in hepatocelluar carcinoma(HCC). [J]. Journal of Chongqing Medical University 34(7):849-851(2009)
[1]吴桂新,王洪林,邓开,郑科,董蒲江.Livin,Survivin在肝癌细胞的表达及5-Fu对其的诱导作用[J].重庆医科大学学报,2009,34(07):849-851. DOI：

WU Gui-xin, et al. The expression of Inhibitor of apoptosis protein of Livin and Survivin induced by 5-Fu in hepatocelluar carcinoma(HCC). [J]. Journal of Chongqing Medical University 34(7):849-851(2009) DOI：

摘要

目的:研究凋亡抑制蛋白Livin

Survivin在肝癌细胞株HepG2的表达及5-Fu对肝癌细胞内凋亡抑制蛋白Livin

Survivin的诱导作用

探讨Livin

Survivin在肝癌化疗抵抗中的作用机制。方法:培养人肝癌细胞株HepG2

加入低浓度的化疗药物5-Fu

分别在加药前、加药后24h和48h采用逆转录聚合酶链反应（RT-PCR）和免疫印迹技术（Western-blot）检测livin

Survivin mRNA和蛋白的表达。结果:给予低浓度的5-Fu可诱导Livin

SurvivinmRNA和蛋白的表达发生变化

其中Livin mRNA在加药后24h和48h分别较加药前提高了0.6倍和1.1倍

Livin蛋白在加药后24h和48h分别较加药前提高了1.3倍和2.7倍

Survivin mRNA在加药后24h和48h分别较未加药提高了1.5倍和2.2倍

Survivin蛋白在加药后24h和48h分别较未加药提高了0.9倍和1.9倍。结论:Livin

Survivin在HepG2中有表达。5-Fu可诱导肝癌细胞内livin

Survivin的表达增加

抵抗化疗药物诱导的细胞凋亡。值得进一步研究以评价5-Fu对肝癌治疗的实用价值。

Abstract

Objective:To investigate the expression of livin and survivin induced by 5-Fu agent

and determine the role of livin and survivin in anti-chemotherapy of hepatocellular carcinoma(HCC). Methods:Low concentration of 5-Fu was added in cultured HepG2 cells.The expression of livin and survivin was detected by RT-PCR and western-blot on 24h and 48h after 5-Fu treatment.Results:Livin and survivin mRNA 1eve1 was increased by 0.6

1.1 and 1.5

2.2 times

respectively.and thant of the protein expression was increased by 1.3

2.7 and 0.9

1.9 times

respectively.Conclusion:livin and survivin was expression in HepG2.A high expression level of livin and survivin was induced in HepG2 by 5-Fu

which reduced to apoptosis by 5-Fu.It’s valuable to study the curative effect of 5-Fu to hepatocelluar carcinoma further.

关键词

肝癌细胞株HepG2livinSurvivin

Keywords

Hepatocarcinoma cell line HepG2livinSurvivin

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