Curcumin reduces Aβgeneration of neurons by PPARγ activation and BACE1 inhibition

SI Lu; et al

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Curcumin reduces Aβgeneration of neurons by PPARγ activation and BACE1 inhibition

更新时间：2024-01-17

- Curcumin reduces Aβgeneration of neurons by PPARγ activation and BACE1 inhibition
- Journal of Chongqing Medical University Vol. 34, Issue 10, Pages: 1300-1304(2009)
- 作者机构：
  
  1. 重庆医科大学病理教研室
  2. 重庆医科大学神经科学研究中心
- 作者简介：
- 基金信息：
- DOI：
  CLC： R749.16
- Published：2009，
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[1]斯露,张雄,张红梅,李昱.姜黄素通过激活PPARγ和抑制BACE1减少神经细胞内β淀粉样蛋白的产生[J].重庆医科大学学报,2009,34(10):1300-1304.

SI Lu, et al. Curcumin reduces Aβgeneration of neurons by PPARγ activation and BACE1 inhibition. [J]. Journal of Chongqing Medical University 34(10):1300-1304(2009)
[1]斯露,张雄,张红梅,李昱.姜黄素通过激活PPARγ和抑制BACE1减少神经细胞内β淀粉样蛋白的产生[J].重庆医科大学学报,2009,34(10):1300-1304. DOI：

SI Lu, et al. Curcumin reduces Aβgeneration of neurons by PPARγ activation and BACE1 inhibition. [J]. Journal of Chongqing Medical University 34(10):1300-1304(2009) DOI：

摘要

目的:本课题旨在研究姜黄素处理体外培养的神经细胞后BACE1、PPARγ及Aβ40和Aβ42的变化

探讨其抑制β淀粉样蛋白作用的可能机制。方法:通过LipofectaminTM2000将质粒APPswe与BACE1-mychis瞬时共转染入SHSY5Y细胞

然后用姜黄素分浓度组与时间组处理细胞

通过RT-PCR测定BACE1及PPARγ的mRNA水平;通过Western Blotting检测BACE1及PPARγ的蛋白表达;通过ELISA检测Aβ40和Aβ42的变化。结果:RT-PCR及Western blot结果显示转染细胞经姜黄素处理后BACE1的mRNA及蛋白表达水平显著减少

呈浓度和时间依赖性（P<0.05）;而PPARγ的mRNA及蛋白表达水平增加

也呈浓度和时间依赖性（P<0.05）。ELISA结果显示Aβ40和Aβ42的产生随处理浓度及时间的增加显著降低（P<0.05）。结论:姜黄素可显著抑制体外培养的神经元中Aβ40和Aβ42的产生

且该作用与其激活PPARγ和抑制BACE1密切相关。

Abstract

Objective: To investigate the inhibitory effects of Curcumin on Aβ generation in vitro and explore its potential mechanisms involing BACE1 and PPARγ.Methods: Plasmids APPswe and BACE1-mychis were transiently co-transfected into SHSY5Y cells by LipofectaminTM2000

then treated with Curcumin at 0(control Group)

1.25

5.0

20.0 μmol/L for 24 hr

or with Curcumin at 5 μmol/L for 0

24 and 48 hrs for the time course assay.RT-PCR was performed to measure the endogenous levels of BACE1 mRNA and PPARγ.Western blot were used to detect the protein expression of BACE1 and PPARγ.The major cleavage product——Aβ40 and Aβ42 was detected by β-amyloid 1-40 or 42 Colorimetric ELISA assay.Results: The mRNA level and protein level of BACE1 were decreased obviously in a dose-and time-dependent manner after treated with Curcumin in the cell line(P<0.05).Conversely

the expression of PPARγ increased at both mRNA and protein levels after treated with curcumin

also they were in a dose-and time-pendent manner(P<0.05).ELISA results showed that the generation of Aβ40 and Aβ42 reduced significantly

also in a dose-and time-dependent manner(P<0.05).Conclusion: Aβ generation could be obviously inhibited by Curcumin in co-transfected SHSY5Y cell lines and that the protective mechanism of Curcumin in AD involves activation of PPARγ and decreasing BACE1 at gene and protein levels.

关键词

阿尔茨海默病姜黄素PPARγBACE1Aβ

Keywords

Alzheimer’s diseasecurcuminPPARγBACE1Aβ

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