The mechanism investigation of the reactive oxygen species and glutathione changes in the process of K562 cells induced by simvastatin

ZENG Ya-li; et al

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The mechanism investigation of the reactive oxygen species and glutathione changes in the process of K562 cells induced by simvastatin

更新时间：2024-01-17

- The mechanism investigation of the reactive oxygen species and glutathione changes in the process of K562 cells induced by simvastatin
- Journal of Chongqing Medical University Vol. 34, Issue 8, Pages: 992-995(2009)
- 作者机构：
  
  1. 四川绵阳四○四医院检验科
  2. 四川省医学科学院·四川省人民医院检验科
  3. 重庆医科大学医学检验系
- 作者简介：
- 基金信息：
- DOI：
  CLC： R733.7
- Published：2009，
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[1]曾娅莉,黄文芳,杨永长,邓建军,何静,昔国强,刘文.活性氧与谷胱甘肽在辛伐他汀诱导K562细胞凋亡时的变化及机制探讨[J].重庆医科大学学报,2009,34(08):992-995.

ZENG Ya-li, et al. The mechanism investigation of the reactive oxygen species and glutathione changes in the process of K562 cells induced by simvastatin. [J]. Journal of Chongqing Medical University 34(8):992-995(2009)
[1]曾娅莉,黄文芳,杨永长,邓建军,何静,昔国强,刘文.活性氧与谷胱甘肽在辛伐他汀诱导K562细胞凋亡时的变化及机制探讨[J].重庆医科大学学报,2009,34(08):992-995. DOI：

ZENG Ya-li, et al. The mechanism investigation of the reactive oxygen species and glutathione changes in the process of K562 cells induced by simvastatin. [J]. Journal of Chongqing Medical University 34(8):992-995(2009) DOI：

摘要

目的:研究辛伐他汀（simvastatin）诱导K562细胞凋亡过程中细胞内活性氧与谷胱甘肽的变化及凋亡中发生的时间顺序

以探讨辛伐他汀诱导K562凋亡的机制。方法:20μmol/L浓度辛伐他汀处理K562细胞

48h光学显微镜观察细胞形态学。12、24、48、72hMTT法检测细胞活力

荧光染料流式细胞术检测细胞凋亡率和细胞内活性氧

化学比色法检测细胞内谷胱甘肽。结果:辛伐他汀作用K562细胞48h后出现典型的凋亡形态学改变;12、24、48、72h细胞抑制率为0、（19.02±0.92）%、（56.4±3.20）%、（74.4±5.54）%

K562细胞生长被抑制;细胞凋亡率升高为:（2.55±0.25）%、（6.1±0.35）%、（14.15±0.42）%、（30.70±0.65）%;活性氧的荧光强度升高为:1.61±0.15、13.1±1.54、10.0±1.05、8.10±0.87

与不同时间的对照组相比较均显著升高（P<0.05）。细胞内谷胱甘肽含量为:274.7±11.5、262.5±9.8、272.4±10.5、（357.2±16.8）mg/gprot

与不同时间的对照组相比较均显著降低（P<0.05）。结论:辛伐他汀诱导K562细胞凋亡时细胞内发生了氧化还原体系失衡

它可能是细胞凋亡的早期上游事件。

Abstract

Objective:To investigate the changes of reactive oxygen species(ROS)and Glutathione(GSH) and their chronological order in the apoptosis of K562 cells induced by simvastatin to explore the apoptosis mechanism.Methods:K562 cells cultivated in routine method for 24h were exposed in the simvastatin 20μmol/L.Cell morphological analysis were conducted at 48h and the vitalities of K562 cells were cultivated detected with MTT at different times(12

72 h) respectively.Fluorochrome flow cytometry was performed to detect cell apoptotic ratio and changes of intracellular ROS.The content of intracellular GSH in K562 cells was measured by colorimetric method.Results:K562 cells presented typical apoptosis morphological changes the treatment of simvastatin for 48h.cell inhibition ratio at different times(12

and 72 h) were 0

(19.02±0.92)%

(56.4±3.20)%

(74.4±5.54)% respectively and cell growth of K562 was inhibited.The apoptotic ratio were(2.55±0.25)%

(6.1±0.35)%

(14.15±0.42)%

and(30.70±0.65)% respectively.Furthermore

the changes of fluorescence intensity of ROS were also significantly increased(P<0.05) in K562 cells compared with the control groups

the changes of fluorescence intensity were 1.61±0.15

13.1±1.59

10.0±1.05

and 8.10±0.87mg/gprot respectively groups at different times(12

48 and 72h).The content of intracellular GSH in K562 cells GSH were 274.7±11.5

262.5±9.8

272.4±10.5

and 357.2±16.8 mg/gprot at different times(12

48 and 72 h) respectively.And there was a markedly decrease of the intracellular GSH in K562 cells compared with the control groups(P<0.05).Conclusion:K562 cells could be induced apoptosis by simvastatin.The increase of the GSH and decrease of the ROS are the early events of the K562 cells apoptosis induced by simvastatin.The potential mechanism of the K562 cells apoptosis may be related to the disequilibrium of oxidation-reduction system.

关键词

辛伐他汀细胞凋亡活性氧谷胱甘肽

Keywords

simvastatinapoptosisreactive oxygen speciesglutathione

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