Objective:hepaCAM (hepatocyte cell adhesion molecule)is a kind of immunoglobulin superfamily member which was discovered as a tumor suppressor. This study was to investigate the effects of hepaCAM transfection on proliferation and invasion of human renal cancer cell line 786-0. Methods:An eukaryotic expression plasmid containing hepaCAM

hepaCAM-pEGFPN2 was transfected into 786-0 cells

then positive cell clones were selected with G418 and amplified. pEGFPN2 transfected cells and untreated cells were set as controls.The expression of hepaCAM was detected by reverse transcription-polymerase chain reaction (RT-PCR). Proliferative ability of 786-0 cells was measured by MTT assay and the invasive ability in vitro was determined by transwell chambers method. Results: Plasmid pEGFPN2

hepaCAM-pEGFPN2 were successfully constructed and stably transfected into 786-0 cells.After transfection

the inhibitory rates of cell growth at the fourth、 fifth and sixth day were 26.5%

38.1% and 35.7%

respectively. The penetrating cells were significantly less in 786-0/ hepaCAM-pEGFPN2 group than in 786-0/pEGFPN2 and 786-0 groups (24.6±5.3vs35.5±6.2 and 37.1±7.0

P<0.01). Conclusion:Transfection with hepaCAM might suppress proliferative and invasive ability of renal cancer 786-0 cells.