Detection and sequencing of different subtypes of HPV in laryngeal carcinoma

PENG Fang-yi; et al

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Detection and sequencing of different subtypes of HPV in laryngeal carcinoma

更新时间：2024-01-17

- Detection and sequencing of different subtypes of HPV in laryngeal carcinoma
- Journal of Chongqing Medical University Vol. 34, Issue 12, Pages: 1689-1692(2009)
- 作者机构：
  
  1. 重庆理工大学化学与生物工程学院制药工程系
  2. 重庆市江北区第一人民医院检验科
  3. 重庆医科大学图书馆文献检索教研室
  4. 重庆市第四人民医院妇产科
  5. 重庆市第四人民医院麻醉科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R739.65
- Published：2009，
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[1]彭方毅,姜海蓉,刘芳,杜志银,林治华,彭方亮,赵卫兵.喉癌中HPV不同亚型的检测及测序[J].重庆医科大学学报,2009,34(12):1689-1692.

PENG Fang-yi, et al. Detection and sequencing of different subtypes of HPV in laryngeal carcinoma. [J]. Journal of Chongqing Medical University 34(12):1689-1692(2009)
[1]彭方毅,姜海蓉,刘芳,杜志银,林治华,彭方亮,赵卫兵.喉癌中HPV不同亚型的检测及测序[J].重庆医科大学学报,2009,34(12):1689-1692. DOI：

PENG Fang-yi, et al. Detection and sequencing of different subtypes of HPV in laryngeal carcinoma. [J]. Journal of Chongqing Medical University 34(12):1689-1692(2009) DOI：

摘要

目的:检测人乳头瘤病毒（Human papillomavirus

HPV）各型DNA在喉癌组织中的表达

探讨HPV在喉癌发生中的作用。方法:用PCR检测123例喉癌组织及123例喉粘膜上皮组织中HPV各型DNA。同时进行DNA测序

结果:在123例喉癌标本中

HPV-L1

-16E6/E7

-18E6/E7阳性率都明显高于其他各型。123例喉粘膜PCR结果表明:低危型HPV的阳性率与喉癌相近;高危型HPV的阳性率虽远远低于喉癌

但随着组织病变程度加重阳性率逐渐升高;HPV-L1的阳性率高于HPV-16E6、E7

随着组织病变程度加重

HPV-L1的检出率逐渐增高。结论:HPV-16感染会增强喉上皮病变的风险

而HPV-18有协同作用;L1片段适合用于检测HPV感染。

Abstract

Objective:To look for the further evidence for different subtypes of HPV as carcinogenic factors in laryngeal carcinoma.Methods:We examined representative numbers of specimens from laryngeal cancer with highly sensitive PCR technique and sequencing for the presence of different subtypes of HPV.Results:The positive rates of HPV-L1

-16E6/E7

-18E6/E7 were significantly higher than other types in 123 cases of laryngeal carcinoma specimens.PCR was used to screen the prevalence of HPV infection.The prevalence of HPV-16E6 was lower than laryngeal carcinoma.But

as the cells undergoing pathological progress

the HPV-16E6 positive rate was increased.The prevalence of HPV-16E7 was similar to the prevalence of HPV-16E6.The positive rate of HPV-L1 was the highest and the prevalence of HPV-L1 was significantly increased as the cells underwent pathological progress.Conclusion:HPV-16 and HPV-18 may synergistically function on the pathogenesis of laryngeal epithelium.L1 segment is fit for detecting HPV infection.

关键词

人乳头瘤病毒聚合酶链反应序列测定喉癌

Keywords

HPVPolymerase chain reactionSequencinglaryngeal carcinoma

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重庆医科大学基础医学院微生物学教研室重庆医科大学基础医学院微生物学教研室讲师

重庆医科大学基础医学院微生物学教研室讲师

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