Effects of transplanting mesenchymal stem cells to hippocampus on PSA-NCAM in DM rats

XIA li; et al

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Effects of transplanting mesenchymal stem cells to hippocampus on PSA-NCAM in DM rats

更新时间：2024-01-17

- Effects of transplanting mesenchymal stem cells to hippocampus on PSA-NCAM in DM rats
- Journal of Chongqing Medical University Vol. 34, Issue 11, Pages: 1487-1490(2009)
- 作者机构：
  
  重庆医科大学附属第一医院老年科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R587.1
- Published：2009，
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[1]夏丽,卢卫琴,赵柯湘,高原,李娟,肖谦.间充质干细胞海马移植对糖尿病大鼠PSA-NCAM的影响[J].重庆医科大学学报,2009,34(11):1487-1490.

XIA li, et al. Effects of transplanting mesenchymal stem cells to hippocampus on PSA-NCAM in DM rats. [J]. Journal of Chongqing Medical University 34(11):1487-1490(2009)
[1]夏丽,卢卫琴,赵柯湘,高原,李娟,肖谦.间充质干细胞海马移植对糖尿病大鼠PSA-NCAM的影响[J].重庆医科大学学报,2009,34(11):1487-1490. DOI：

XIA li, et al. Effects of transplanting mesenchymal stem cells to hippocampus on PSA-NCAM in DM rats. [J]. Journal of Chongqing Medical University 34(11):1487-1490(2009) DOI：

摘要

目的:探讨间充质干细胞在糖尿病大鼠海马的移植对多唾液酸-神经细胞粘附分子（Polysialylated acid-nural cell adhesion molecule

PSA-NCAM）的影响。方法:将30只SD大鼠随机分为正常对照组、糖尿病组、干细胞移植组。建立STZ糖尿病大鼠模型。原代培养大鼠骨髓间质干细胞

分离纯化鉴定后移植入糖尿病大鼠海马部位

杀鼠取海马

用PSA-NCAM单克隆抗体及采用RT-PCR的方法检测海马中PSA-NCAM的水平

并与糖尿病对照组及正常组加以比较。结果:移植组与糖尿病组比较

PSA-NCAM蛋白和mRNA（P=0.0137）表达均增加

但显著低于正常组（P=0.015）。结论:间充质干细胞海马移植增加海马中PSA-NCAM的表达。

Abstract

Objective:To investigate the influence of transplantation with MSCs to hippocampus on PSA-NCAM in DM rats.Methods:30 SD rats were divided into the normal control group

diabetic model group

and stem cells transplanted group.Streptozotocin-diabetic rat model was established.MSCs were separated from bone marrow of rats.After purified

they were transplanted to hippocampus of DM rats.Then

the rats were Secrificed

to get the hippocampus for detect in of the expression of PSA-NCAM by immunofluorescence and RT-PCR.the data of the three groups were Compared.Results:Compared with diabetic model group

the transplanted group showed a significant increase in the expression of PSA-NCAMprotein and mRNA in hippocampus(P <0.05)

butthey were significantly lower than those in normal control group(P<0.05).Conclusion:Transplantation with stem cells could increase the expression of PSA-NCAM in hippocampus of DMrats.

关键词

糖尿病海马间充质干细胞多唾液酸-神经细胞粘附分子

Keywords

diabetes mellitushippocampusBone marrow mesenchymal stem cellsPolysialylated acid-nural cell adhesion molecule

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