Effect of sivelestat sodium hydrate on proteomics in rats with global cerebral ischemia/reperfusion

HU Yue; et al

您当前的位置：

首页 >

文章列表页 >

Effect of sivelestat sodium hydrate on proteomics in rats with global cerebral ischemia/reperfusion

更新时间：2024-01-17

- Effect of sivelestat sodium hydrate on proteomics in rats with global cerebral ischemia/reperfusion
- Journal of Chongqing Medical University Vol. 34, Issue 12, Pages: 1622-1625(2009)
- 作者机构：
  
  重庆医科大学生物化学与分子药理学重点实验室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R741
- Published：2009，
扫描看全文
[1]胡月,董志.西维来司钠对脑缺血/再灌注大鼠全脑蛋白质组的影响[J].重庆医科大学学报,2009,34(12):1622-1625.

HU Yue, et al. Effect of sivelestat sodium hydrate on proteomics in rats with global cerebral ischemia/reperfusion. [J]. Journal of Chongqing Medical University 34(12):1622-1625(2009)
[1]胡月,董志.西维来司钠对脑缺血/再灌注大鼠全脑蛋白质组的影响[J].重庆医科大学学报,2009,34(12):1622-1625. DOI：

HU Yue, et al. Effect of sivelestat sodium hydrate on proteomics in rats with global cerebral ischemia/reperfusion. [J]. Journal of Chongqing Medical University 34(12):1622-1625(2009) DOI：

摘要

目的:研究西维来司钠（Sivelestat sodium hydrate

ONO-5046）对脑缺血/再灌注（Cerebral ischemia/reperfusion

CI/R）大鼠全脑蛋白质表达的影响。方法:大鼠随机分为假手术组、CI/R组和CI/R+ONO-5046组（6mg/kg）。采用四血管阻断法制备CI/R模型

缺血15min

再灌注24h。提取脑组织总蛋白进行双向电泳

对差异蛋白点用HPLC-Chip-MS/MS纳流液质联用技术进行序列分析

经Spectrum Mill搜索NCBInr数据库后鉴定蛋白质。结果:成功获得了分辨率和重复性好的大鼠脑组织蛋白2-DE图谱

经质谱鉴定最终获得了巨噬细胞炎性蛋白1-beta、白细胞弹性蛋白酶A、α2-巨球蛋白、谷胱甘肽S-转移酶omega-1、谷氨酸脱氢酶-1、细胞色素C还原酶铁硫亚单位、硫氧还蛋白过氧化物酶1等19个差异蛋白点相关信息。结论:大鼠脑缺血后脑组织蛋白质表达存在明显差异

西维来司钠可通过调节有关蛋白质的表达

参与大鼠脑缺血神经元保护作用。

Abstract

Objective:Tostudy the effect of Sivelestat sodium hydrate(ONO-5046)on brain proteomics in rats with cerebral ischemia/ reperfusion(CI/R).Methods:SD rats were randomly divided into sham operated group

CI/R group and CI/R+ONO-5046 group(6 mg/kg).CI/R was induced by four-vessel occlusion method

with ischemia for 15 min

and reperfusion 24 h.After 2-dimensional electrophoresis and PDQuest for gel image analysis

analysis

the MS/MS of the peptides from the differential proteins digested by tripsin were analyzed by HPLC-Chip-MS/MS and the original data were pre-processed by Spectrum Mill then searched in NCBInr database.Results:Well resolved

reproducible 2-DE profiles of rat brain proteome were obtained.Among the differential protein spots

19 were identified

Such as small inducible cytokine A4

Leukocyte elastase inhibitor A

Alpha-2-macroglobulin

Glutathione S-transferase omega-1

glutamate dehydrogenase-1

Thioredoxin peroxidase 1

Cytochrome b-c1 complex subunit Rieske and so on.Conclusion:The cerebral proteomics expreesion after CI/R has obvious difference

Sivelestat sodium hydrate may promote the survival of injured neurons in rats by virtue of up or down-regulating the expression levels of proteins concerned with cerebral ischemia injury.

关键词

西维来司钠脑缺血蛋白质组学双向电泳

Keywords

Sivelestat sodium hydratecerebral ischemiaproteomicsTwo-dimensional electrophoresis

references

Views

106

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Establishment of a two-dimensional electrophoresis technology on brain tissue protein of rat

Establishment of a two-dimensional electrophoresis technology on colon carcinoma

Expression and function of miR-124 in cerebral ischemia

The neurotrophic effect of the periischemic brain tissue extract on culture neurons Institute of Neurology

Expression of Bak and Bad gene in brain after global ischemia-reperfusion

Related Author

ZHOU Chenglin

et al

李惠芝

邱宗荫

宋敏

MA Guozhong

et al

周成林

Related Institution

Department of Pharmaceutical Analysis,Pharmaceutial School,Chongqing Medical University

重庆医科大学检验系

Department of General Surgery, the Third Hospital of Mianyang

重庆医科大学药学院

Teaching and Research Section of Pathology,College of Basic Medicine,Institute for Neuroscience,Chongqing Medical University,Chongqing Key Laboratory of Neurobiology

⁰