Effect of rosiglitazone on the apoptosis of beta cells in rats with experimental type 1 diabetic mellitus

GAO Hui-min1; MENG Bi-hui2; WEI Jing-bin3; CHEN Hong-liu4; LIN Yuan-yuan5

您当前的位置：

首页 >

文章列表页 >

Effect of rosiglitazone on the apoptosis of beta cells in rats with experimental type 1 diabetic mellitus

更新时间：2024-01-17

- Effect of rosiglitazone on the apoptosis of beta cells in rats with experimental type 1 diabetic mellitus
- Journal of Chongqing Medical University Vol. 36, Issue 11, Pages: 1304-1306(2011)
- 作者机构：
  
  1. 河南省周口市中心医院内分泌科
  2. 广西医科大学第一附属医院内分泌科
  3. 广西柳州市人民医院传染科
  4. 广西医科大学第一附属医院急诊科
  5. 广西南宁市第一人民医院内分泌科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R587.1
- Published：2011，
扫描看全文
[1]高会敏,蒙碧辉,韦静彬,陈洪流,林媛媛.罗格列酮对实验性1型糖尿病大鼠胰岛β细胞凋亡的影响[J].重庆医科大学学报,2011,36(11):1304-1306.

GAO Hui-min1, MENG Bi-hui2, WEI Jing-bin3, et al. Effect of rosiglitazone on the apoptosis of beta cells in rats with experimental type 1 diabetic mellitus. [J]. Journal of Chongqing Medical University 36(11):1304-1306(2011)
[1]高会敏,蒙碧辉,韦静彬,陈洪流,林媛媛.罗格列酮对实验性1型糖尿病大鼠胰岛β细胞凋亡的影响[J].重庆医科大学学报,2011,36(11):1304-1306. DOI：

GAO Hui-min1, MENG Bi-hui2, WEI Jing-bin3, et al. Effect of rosiglitazone on the apoptosis of beta cells in rats with experimental type 1 diabetic mellitus. [J]. Journal of Chongqing Medical University 36(11):1304-1306(2011) DOI：

摘要

目的:观察罗格列酮（Rosiglitazone natrium

RSG）对实验性1型糖尿病大鼠胰岛β细胞凋亡的影响。方法:6周龄SD大鼠随机分成3组

正常对照（Control

Con）组24只

糖尿病（Diabetic mellitus

DM）组24只

罗格列酮干预（Rosiglitazone

RSG）组24只。以链脲佐菌素（Streptozotocin

STZ）溶液单次腹腔注射

72 h后测空腹血糖（Fasting blood glucose

FBG）≥16.7 mmol/L为造模成功。应用罗格列酮后5个时间段（2、5、7、10、13周）

分别用TUNEL（Terminal deoxynucleotidyl transferase dUTP nickend labeling）方法检测胰岛β细胞的凋亡

用免疫组化法检测胰岛中胰岛素（Insulin

Ins）及Fasl的表达。结果:与DM组相比

RSG组胰岛表达胰岛素水平提高

β细胞相对凋亡指数及Fasl表达水平均下降（P均<0.05）。结论:罗格列酮能抑制或延缓β细胞凋亡

其机制可能与抑制Fas/Fasl细胞凋亡通路有关。

Abstract

Objective:To explore the effect of rosiglitazone on the apoptosis of pancreatic beta cells in rats with experimental type 1 diabetic mellitus.Methods:Sprague-Dawley rats were randomly divided into control group(Con

n=24) and diabetic control group(DM

n=24) and rosiglitazone group(RSG

n=24).Diabetic rats were induced by streptozotocin(STZ) injected intraperitoneally.Rats with fasting blood glucose(FBG)≥16.7 mmol/L were served as diabetic model.Two-

five-seven-

ten-

and thirteen weeks after RSG treatment

the apoptosis of beta cell was evaluated by Terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL) assay and the levels of expression for insulin and Fasl were performed by immunohistochemistry.Results:Two weeks after RSG treatment

the levels of expression for insulin in the pancreas islet were higher(P<0.05) and the relative index of apoptosis of beta cells was lower(P<0.05) coupling a downgraded expression of Fasl(P<0.05) in RSG than that in DM.Conclusion:Rosiglitazone can inhibit or down regulating the apoptosis of beta cells to some extent in experimental type 1 diabetic rats

and one of the mechanisms was probably through inhibiting the apoptosis pathway of Fas/Fasl.

关键词

罗格列酮细胞凋亡糖尿病Fas/Fasl胰岛β细胞

Keywords

rosiglitazoneapoptosisdiabetes mellitusFas/Faslpancreatic beta cell

references

Views

109

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Glycyrrhiza uralensis Fisch. endophytes combined with cisplatin on proliferation and apoptosis of A549 cells

Milk fat globule-EGF factor Ⅷ attenuates apoptosis and permeability damage of the intestinal mucosa in rats with obstructive jaundice

Effect of andrographolide on TBHP-induced apoptosis of intervertebral disc nucleus pulposus cells and its mechanism

Protection of sodium glucose cotransporter-2 inhibitors on diabetic nephropathy

Research progress of ghrelin and diabetes mellitus

Related Author

Zhang Shanglong

Zhang Nan

Lian Xiaolong

Ye Liqiao

Ma Quhuan

Deng Yi

Guo Yong

Tang Yongliang

Related Institution

College of Pharmacy，Gansu University of Chinese Medicine

Department of Hepatobiliary Pancreatic Surgery,Daping Hospital,Army Medical University

Department of Hepatobiliary Pancreatic Surgery,People’s Hospital of Chongqing Liang Jiang New Area

Department of Orthopedics，Wuhan Third Hospital

Department of Endocrinology,The First Affiliated Hospital of Chongqing Medical University

⁰