Effect of HCG-stimulation on in-vitro maturation of immature mouse oocytes

WANG Lian-lian; QIU Yan; XING Gui-bang

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Effect of HCG-stimulation on in-vitro maturation of immature mouse oocytes

更新时间：2024-01-17

- Effect of HCG-stimulation on in-vitro maturation of immature mouse oocytes
- Journal of Chongqing Medical University Vol. 36, Issue 11, Pages: 1322-1324(2011)
- 作者机构：
  
  重庆医科大学附属第一医院辅助生殖中心
- 作者简介：
- 基金信息：
- DOI：
  CLC： R714.8
- Published：2011，
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[1]王炼炼,丘彦,幸贵邦.HCG刺激对未成熟卵母细胞体外成熟培养的影响[J].重庆医科大学学报,2011,36(11):1322-1324.

WANG Lian-lian, QIU Yan, XING Gui-bang. Effect of HCG-stimulation on in-vitro maturation of immature mouse oocytes. [J]. Journal of Chongqing Medical University 36(11):1322-1324(2011)
[1]王炼炼,丘彦,幸贵邦.HCG刺激对未成熟卵母细胞体外成熟培养的影响[J].重庆医科大学学报,2011,36(11):1322-1324. DOI：

WANG Lian-lian, QIU Yan, XING Gui-bang. Effect of HCG-stimulation on in-vitro maturation of immature mouse oocytes. [J]. Journal of Chongqing Medical University 36(11):1322-1324(2011) DOI：

摘要

目的:探讨获取未成熟卵母细胞之前使用人绒毛膜促性腺激素（Human chorionic gonadotropin

HCG）能否改善未成熟卵母细胞在体外培养条件下成熟的时间、体外受精率、2细胞、4细胞发生率。方法:将HCG刺激后或未刺激所获得的小鼠生殖泡（Germinal reside

GV）期卵丘-卵母细胞复合物在培养液中进行体外培养

观察未成熟卵母细胞减数分裂的恢复、核成熟、受精率以及胚胎的发育。结果:体外培养后16 h G-PR组生殖泡破裂（Germinal vesicle break down

GVBD）发生率显著高于G-UPR组（P<0.01）。体外培养后20 h G-PR组GVBD发生率明显高于G-UPR组（P<0.05）。体外培养后24 h G-PR组PBI发生率显著高于G-UPR组（P<0.01）。G-PR组受精率明显高于G-UPR组（P<0.05）。结论:HCG加快了卵母细胞的体外成熟进程

同时也增加了卵母细胞的体外受精率。

Abstract

Objective:To investigate the in-vitro maturation time

in vitro fertilization

2 cell and 4 cell rate of the immature oocytes after the use of HCG(human chorionic gonadotropin).Methods:The germinal vesicle(GV) immature oocytes were acquired from the ovaries of the HCG(human chorionic gonadotropin)-stimulated and non-HCG-stimulated rats.Immature oocytes were cultured in culture media.Observations were made for the status of germinal vesicle break-down(GVBD) and the extrusion of the first polar body(PB1).The achieved MⅡoocytes were then inseminated in-vitro;and the pronuclei(PN) and the pre-implantation development were observed.Results:Observations showed that after being cultured via in-vitro maturation in culture media for 16 hours

the GVBD in the GV cumulus-oocyte complexes(COC) occurring in the HCG-stimulated group had better outcomes than in non-HCG-stimulated group(P<0.01).After being cultured for 20 hours the GVBD occurring in the HCG-stimulated group had better outcomes than in non-HCG-stimulated group(P<0.01).After being cultured for 24 hours

the elimination ratio of PB1 in the HCG-stimulated group was higher than that of the non-HCG-stimulated group(P<0.01).The fertilization rate of the oocytes in the HCG-stimulated group was higher than that in non-HCG-stimulated group(P<0.05).Conclusion:Stimulation with HCG can speed up the maturation of the oocytes

and enhance the fertilization rate.

关键词

绒促性腺激素卵母细胞体外成熟

Keywords

human chorionic gonadotropinoocytein-vitro maturation

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