Preparation of pshRNA-survivin liposome and preliminary study on its stability

LI Yao-wei1; SUN Jiang-chuan1; CHANG Shu-fang1; ZHANG Yong1; CHEN Jia1; WANG Zhi-gang2

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Preparation of pshRNA-survivin liposome and preliminary study on its stability

更新时间：2024-01-17

- Preparation of pshRNA-survivin liposome and preliminary study on its stability
- Journal of Chongqing Medical University Vol. 36, Issue 10, Pages: 1211-1214(2011)
- 作者机构：
  
  1. 重庆医科大学附属第二医院妇产科
  2. 重庆医科大学附属第二医院超声影像研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC： R943
- Published：2011，
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[1]李耀威,孙江川,常淑芳,张勇,陈佳,王志刚.pshRNA-survivin脂质体的制备及初步稳定性考察[J].重庆医科大学学报,2011,36(10):1211-1214.

LI Yao-wei1, SUN Jiang-chuan1, CHANG Shu-fang1, et al. Preparation of pshRNA-survivin liposome and preliminary study on its stability. [J]. Journal of Chongqing Medical University 36(10):1211-1214(2011)
[1]李耀威,孙江川,常淑芳,张勇,陈佳,王志刚.pshRNA-survivin脂质体的制备及初步稳定性考察[J].重庆医科大学学报,2011,36(10):1211-1214. DOI：

LI Yao-wei1, SUN Jiang-chuan1, CHANG Shu-fang1, et al. Preparation of pshRNA-survivin liposome and preliminary study on its stability. [J]. Journal of Chongqing Medical University 36(10):1211-1214(2011) DOI：

摘要

目的:制备包封率高、粒径均匀、稳定性好的pshRNA-survivin脂质体

并对其质量进行评价。方法:采用逆相蒸发法制备pshRNA-survivin脂质体;正交设计优选制备处方;考察脂质体的粒径分布、zeta电位;用超滤离心法测定脂质体的包封率;离心加速实验与渗漏率的测定考察脂质体的稳定性。结果:所得优化处方为磷脂与药物比为100∶1

油水比为4∶1

磷脂与胆固醇之比为1.5∶1

旋转蒸发温度为37℃;以优化处方制得的脂质体平均粒径为123.4 nm

zeta电位为（-8.42±0.88）mV

平均包封率达77.9%。结论:选用逆相蒸发法优化pshRNA-survivin脂质体处方合理

工艺简便可行

包封率高

稳定性好。

Abstract

Objective:To prepare pshRNA-survivin liposome with high entrapment efficiency and good stability

and evaluate its quality.Methods:pshRNA-survivin liposome was prepared by the reversed-phase evaporation method.The optimum formation was selected by means of orthogonal design of experiment;particle size

zeta potential and stability of the liposome were evaluated.Entrapment efficiency was determined by centrifugal ultrafiltration.Centrifugal acceleration experiment and determination of leak rate were served to prove the liposome stability.Results:The optimum formula was as follows:the ratio of lecithin to pshRNA-survivin was 100∶1;oil-water ratio was 4∶1;lecithin:cholesterol was 1.5∶1;temperature of steaming was 37℃;the average diameter and zeta potential of pshRNA-survivin liposome were 123.4 nm and(-8.42±0.88) mV.The average entrapment efficiency for pshRNA-survivin was 77.9%.Conclusion:The selected formulation and preparation technique of pshRNA-survivin liposome is reasonable in prescription

practicable in techniques

high in encapsulation efficiency and good in stability.

关键词

pshRNA-survivin脂质体正交设计超滤离心法包封率稳定性

Keywords

pshRNA-survivin liposomeorthogonal designcentrifugal ultrafiltrationencapsulation efficiencystability

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