Influence of silencing clusterin by RNAi on radiosensitivity of human ovarian carcinoma SKOV3 cell-line

HUANG Luo1; ZHANG Xing-ping1; CHEN Cheng1; LUO Qian2; WU Yong-zhong2

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Influence of silencing clusterin by RNAi on radiosensitivity of human ovarian carcinoma SKOV3 cell-line

更新时间：2024-01-17

- Influence of silencing clusterin by RNAi on radiosensitivity of human ovarian carcinoma SKOV3 cell-line
- Journal of Chongqing Medical University Vol. 36, Issue 11, Pages: 1316-1318(2011)
- 作者机构：
  
  1. 重庆医科大学附属第一医院肿瘤科
  2. 重庆市肿瘤研究所放疗科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.31
- Published：2011，
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[1]黄锣,张幸平,陈诚,罗茜,吴永忠.RNAi沉默clusterin基因对卵巢癌SKOV3细胞放射敏感性的影响[J].重庆医科大学学报,2011,36(11):1316-1318.

HUANG Luo1, ZHANG Xing-ping1, CHEN Cheng1, et al. Influence of silencing clusterin by RNAi on radiosensitivity of human ovarian carcinoma SKOV3 cell-line. [J]. Journal of Chongqing Medical University 36(11):1316-1318(2011)
[1]黄锣,张幸平,陈诚,罗茜,吴永忠.RNAi沉默clusterin基因对卵巢癌SKOV3细胞放射敏感性的影响[J].重庆医科大学学报,2011,36(11):1316-1318. DOI：

HUANG Luo1, ZHANG Xing-ping1, CHEN Cheng1, et al. Influence of silencing clusterin by RNAi on radiosensitivity of human ovarian carcinoma SKOV3 cell-line. [J]. Journal of Chongqing Medical University 36(11):1316-1318(2011) DOI：

摘要

目的:研究clusterin（CLU）基因沉默对卵巢癌SKOV3细胞放射敏感性的影响。方法:靶向CLU基因的siRNA转染卵巢癌SKOV3细胞

Western blot法测定CLU基因蛋白表达水平。给予不同放射剂量（2、4、6 Gy）处理后

通过MTT法及克隆形成实验检测卵巢癌SKOV3细胞的存活率及克隆形成率

流式细胞仪Annexin V/PI法检测凋亡率。结果:靶向CLU基因的siRNA特异性地从蛋白水平抑制了CLU基因表达。MTT及克隆形成实验结果显示卵巢癌SKOV3细胞的存活率及克隆形成率与放射剂量呈反比

剂量越大

存活率及克隆形成率越低

差异均具有统计学意义（P<0.05）。流式细胞仪Annexin V/PI法检测结果表明:2 Gy放疗后实验组细胞凋亡率明显增加

达（24.32±1.75）%

较单纯放射组升高约12%

差异具有明显统计学意义（P<0.05）。结论:CLU基因沉默可以明显增强卵巢癌SKOV3细胞对放射的敏感性

为卵巢癌放疗增敏基础研究提供了新的靶点。

Abstract

Objective:To investigate the influence of silencing clusterin(CLU) by RNA interference on radiosensitivity of human ovarian carcinoma SKOV3 cell-line.Methods:The expression of CLU gene was silenced by siRNA against it

Western blot was performed to detect the expression of CLU on protein level.After receiving different doses of radiation(2

6 Gy)

The cell survival rate was detected by MTT assay

and the cloning efficiency was also evaluated by the assay

the apoptotic rate was detected by flow cytometry.Results:The siRNA target clu specifically down regulated the expression of CLU significantly.The MTT and colony forming results showed that the cell survival and cloning efficiency became significantly lower with the increasing dose of ionizing irradiation

the capacity of proliferation and cloning efficiency was inhibited obviously(P<0.05).The apoptotic rate became significantly higher with the increasing dose of ionizing irradiation

which reached(24.32±1.75)% after 2 Gy(P<0.05)

and increased about 12% compared with control group.Conclusion:The CLU-silencing can significantly enhance the radio-sensitivity of ovarian carcinoma SKOV3 cell-line in vitro

and the CLU gene may be a promising target for basic research of ovarian carcinoma.

关键词

clusterin放射敏感性RNAi卵巢癌

Keywords

clusterinradio-sensitivityRNA interferenceovarian cancer

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