Effects of microenvironment of tongue squamous cell carcinoma on the growth and differentiation of bone marrow-derived dendritic cell

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Effects of microenvironment of tongue squamous cell carcinoma on the growth and differentiation of bone marrow-derived dendritic cell

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- Effects of microenvironment of tongue squamous cell carcinoma on the growth and differentiation of bone marrow-derived dendritic cell
- Journal of Chongqing Medical University Vol. 36, Issue 10, Pages: 1172-1175(2011)
- 作者机构：
  
  重庆医科大学口腔医学院口腔颌面外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R739.8
- Published：2011，
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[1]童熹,李勇,李显,王启明,季平,王涛.舌鳞癌细胞微环境对小鼠髓源性树突状细胞生长分化的影响[J].重庆医科大学学报,2011,36(10):1172-1175.

TONG Xi, LI Yong, LI Xian, et al. Effects of microenvironment of tongue squamous cell carcinoma on the growth and differentiation of bone marrow-derived dendritic cell. [J]. Journal of Chongqing Medical University 36(10):1172-1175(2011)
[1]童熹,李勇,李显,王启明,季平,王涛.舌鳞癌细胞微环境对小鼠髓源性树突状细胞生长分化的影响[J].重庆医科大学学报,2011,36(10):1172-1175. DOI：

TONG Xi, LI Yong, LI Xian, et al. Effects of microenvironment of tongue squamous cell carcinoma on the growth and differentiation of bone marrow-derived dendritic cell. [J]. Journal of Chongqing Medical University 36(10):1172-1175(2011) DOI：

摘要

目的:探讨舌鳞癌微环境对小鼠髓源性树突状细胞生长分化的影响。方法:小鼠骨髓前体细胞

在含重组小鼠粒细胞-巨噬细胞集落刺激因子和重组小鼠白介素-4培养体系中体外培养。实验组在上述培养液中再加入Tca8113培养上清液模拟肿瘤微环境（Tumor microenvironment

TME）;对照组不加Tca8113培养上清液;镜下观察DCs形态

台酚蓝染色法计活细胞数

流式细胞术检测DCs表型（CD86、CD11c、MHC-Ⅱ）表达。结果进行LSD-t统计学分析。结果:①Tca8113微环境下可诱导出具有典型形态及表型树突状细胞;②低剂量Tca8113培养上清液微环境刺激增强刺激DCs分化成熟

中等剂量Tca8113培养上清液微环境不影响成熟DC（Mature DC

mDCs）分化

但能促进骨髓前体细胞分化为未成熟DC（Immature DC

imDC）;高剂量Tca8113培养上清液微环境抑制DCs分化成熟。结论:Tca8113微环境下可成功诱导骨髓前体细胞分化为树突状细胞;舌鳞癌微环境对树突状细胞分化影响的多样性与环境中免疫因子浓度密切相关。

Abstract

Objective:To investigate the effects of microenvironment of tongue squamous cell carcinoma on the growth and differentiation of bone marrow-derived dendritic cell.Methods:Marrow precursor cells were cultured in RPMI1640 medium containing rmGM-CSF and rmIL-4 +either cs-Tca8113 or not.Control groups were cultured without any cs-Tca8113.The morphology of DCs were observed under optical microscope;the number of survive cells was calculated by trypan blue;the phenotypes of cells were analyzed by flow cytometry.All results were analyzed statistically by LSD-t.Results:The cells cultured in tumor microenvironment appeared typical morphotype and phenotype.Microenvironment of low dose cs-Tca8113 stimulated DCs to mature.Microenvironment of medium dose cs-Tca8113 promoted bone marrow precursor to differentiate into imDC.Microenvironment of high dose cs-Tca8113 inhibited DCs to differentiate and mature.Conclusion:Bone marrow precursor cells can be successfully induced into DCs with typical characteristics in the microenvironment of Tca8113.The multiple effects of Tca8113 microenvironment on the growth and differentiation of DCs are closely related to the concentration of cs-Tca8113.

关键词

肿瘤微环境树突状细胞免疫逃逸免疫抑制因子

Keywords

tumor microenvironmentdendritic cellimmune escapeimmune suppressor

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