Spinal cord injury in male rats can impact bacterial infection of reproductive system,and IL-1,TNF-α and SOD expressions

YANG Chun-lin; WANG Ming; DENG Yuan-zhong; TIAN Lu-hai

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Spinal cord injury in male rats can impact bacterial infection of reproductive system,and IL-1,TNF-α and SOD expressions

更新时间：2024-01-17

- Spinal cord injury in male rats can impact bacterial infection of reproductive system,and IL-1,TNF-α and SOD expressions
- Journal of Chongqing Medical University Vol. 36, Issue 11, Pages: 1296-1299(2011)
- 作者机构：
  
  重庆医科大学附属第一医院泌尿外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R651.2
- Published：2011，
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[1]杨春林,王明,邓远忠,田禄海.雄性大鼠脊髓损伤对生殖系细菌感染及IL-1、TNF-α、SOD表达的影响[J].重庆医科大学学报,2011,36(11):1296-1299.

YANG Chun-lin, WANG Ming, DENG Yuan-zhong, et al. Spinal cord injury in male rats can impact bacterial infection of reproductive system,and IL-1,TNF-α and SOD expressions. [J]. Journal of Chongqing Medical University 36(11):1296-1299(2011)
[1]杨春林,王明,邓远忠,田禄海.雄性大鼠脊髓损伤对生殖系细菌感染及IL-1、TNF-α、SOD表达的影响[J].重庆医科大学学报,2011,36(11):1296-1299. DOI：

YANG Chun-lin, WANG Ming, DENG Yuan-zhong, et al. Spinal cord injury in male rats can impact bacterial infection of reproductive system,and IL-1,TNF-α and SOD expressions. [J]. Journal of Chongqing Medical University 36(11):1296-1299(2011) DOI：

摘要

目的:了解脊髓损伤后雄性大鼠前列腺、附睾、睾丸细菌感染及IL-1、TNF-α、SOD蛋白的表达情况。方法:80只SD大鼠采用随机数字法分为对照组（n=40）和实验组（n=40）

采用改良Allen法建立脊髓损伤动物模型

分别喂养1、2、4、8、16 d

2组均于每个时间点摘取8只大鼠的前列腺、附睾、睾丸组织进行细菌培养和免疫组织化学SABC法检测白介素-1（Interleukin-1

IL-1）、肿瘤坏死因子-α（Tumor necrosis factor-α

TNF-α）、超氧化物歧化酶（Superoxide dismutase

SOD）的表达。结果:与对照组相比

实验组前列腺、附睾细菌培养阳性数显著提高（P<0.05）

大肠杆菌的检出率高于其他细菌;睾丸IL-1、TNF-α表达明显增多（P<0.05）

实验组间以术后第4天表达水平最高（P<0.05）;SOD表达无明显差异（P>0.05）。结论:脊髓损伤后大鼠前列腺、附睾易受到以大肠杆菌为主的细菌感染

睾丸炎性反应增强

生殖系抗氧化反应可能很弱。

Abstract

Objective:To study the expressions of IL-1

TNF-α

SOD and bacterial infection in male rats’ reproductive system after spinal cord injury.Methods:80 SD rats were divided into control group(40 rats) and experimental group(40 rats) randomly.Animal models of spinal cord injury were established by ’Allen’ method.Both groups of rats were fed for 1 day

2 days

4 days

8 days

and 16 days respectively.Then 8 rats’ prostates

testes and epididymises in each group were extirpated for bacterial culture and immunohistochemistry by SABC to detect the expressions of proteins(IL-1

TNF-α

SOD) at each time point.Results:Compared with control group

the risk of E.coli infection in prostate and epididymis was increased significantly in the experimental group(P<0.05);the expressions of testicular IL-1 and TNF-α were increased(P<0.05) and the expression level was the highest in the fourth days after surgery(P<0.05).SOD expression showed no significant difference in the whole reproductive system(P>0.05).Conclusion:Prostate and epididymis of the rat after spinal cord injury are easily infected by bacteria(E.coli);inflammatory response was enhanced in testicular

and the antioxidant activity of reproductive system may be very weak.

关键词

脊髓损伤生殖系超氧化物歧化酶白介素-1肿瘤坏死因子-α

Keywords

spinal cord injuryreproductive systemsuperoxide dismutase(SOD)interleukin-1(IL-1)tumor necrosis factor-α(TNF-α)

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