An investigation on mechanism of the TLR4/NF-κB on Hcy to promote the damage of HUVECs

XU Zhi-fang1; YU Hai-jiao2; MA Lin-na2; GONG Hui-hui2; LIANG Yu2; JIANG Yi-deng1

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An investigation on mechanism of the TLR4/NF-κB on Hcy to promote the damage of HUVECs

更新时间：2024-01-17

- An investigation on mechanism of the TLR4/NF-κB on Hcy to promote the damage of HUVECs
- Journal of Chongqing Medical University Vol. 36, Issue 12, Pages: 1409-1412(2011)
- 作者机构：
  
  1. 宁夏医科大学基础医学院病理生理学教研室
  2. 宁夏医科大学检验学院生化教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R363
- Published：2011，
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[1]徐支芳,于海娇,马琳娜,巩慧慧,梁宇,姜怡邓.TLR4/NF-κB信号通路在同型半胱氨酸致内皮细胞损伤中的作用研究[J].重庆医科大学学报,2011,36(12):1409-1412.

XU Zhi-fang1, YU Hai-jiao2, MA Lin-na2, et al. An investigation on mechanism of the TLR4/NF-κB on Hcy to promote the damage of HUVECs. [J]. Journal of Chongqing Medical University 36(12):1409-1412(2011)
[1]徐支芳,于海娇,马琳娜,巩慧慧,梁宇,姜怡邓.TLR4/NF-κB信号通路在同型半胱氨酸致内皮细胞损伤中的作用研究[J].重庆医科大学学报,2011,36(12):1409-1412. DOI：

XU Zhi-fang1, YU Hai-jiao2, MA Lin-na2, et al. An investigation on mechanism of the TLR4/NF-κB on Hcy to promote the damage of HUVECs. [J]. Journal of Chongqing Medical University 36(12):1409-1412(2011) DOI：

摘要

目的:Toll样受体4（Toll-like receptor-4

TLR4）/核因子-κB（Nuclear factor kappa B

NF-κB）在同型半胱氨酸（Homo-cysteine

Hcy）致动脉粥样硬化（Atherosclerosis

AS）过程中损伤内皮细胞的作用机制。方法:体外培养原代人脐静脉内皮细胞（Human umbilical vein endothelial cells

HUVECs）

用0、50、100、200、500μmol/L Hcy和100μmol/L Hcy+30μmol/L（维生素B12+叶酸）分别与HUVECs培养72 h后

采用MTT法观察Hcy对HUVECs活性的影响;用分光光度比色法检测各组HUVECs中丙二醛（Malondialdehyde

MDA）和超氧化物歧化酶（Superoxide dismutase

SOD）;酶免法检测NF-κB;荧光定量PCR和Westernblot分别测TLR4 mRNA和蛋白表达。结果:Hcy可抑制HUVECs的活性

各Hcy干预组与对照组比较细胞活性均降低

其中100、200、500μmol/L Hcy组与对照组比较差异有统计学意义（P<0.05）

而其他组与对照组比较差异无统计学意义（P>0.05）;随Hcy浓度增加MDA和NF-κB含量和TLR4 mRNA和蛋白表达明显增加

而各实验组SOD活性明显降低

其中

100、200、500μmol/L Hcy组与对照组比较差异有统计学意义（P<0.05）

其他组与对照组比较差异无统计学意义（P>0.05）。结论:TLR4/NF-κB可能是Hcy致HUVECs损伤的重要机制之一

其中MDA和SOD起了重要的作用。

Abstract

Objective:To investigate the mechanism of TLR4/NF-κB on Hcy to promote the damage of the vein endothelial cells on plaques of atherosclerosis.Methods:Cultured HUVECs were treated by 0

100

200 μmol/L and 500 μmol/L Hcy

100 μmol/L Hcy+30 μmol/L(VitaminB12+Folic acid) concentrations of Hcy for 72 h.The activity of HUVECs was examined by MTT assay.The MDA and NF-κB contents and SOD activity were measured by chromatometry and ELISA.The mRNA and protein expressions of TLR4 were detected by fluorescent quantitation PCR and Western blot.Results:MTT test showed that Hcy could restrain the activity of HUVECs after being treated by Hcy for 72 h.The activity of 100 to 500 μmol/L Hcy compared to control cells were significant(P<0.05)

but other cells compared to control cells were not significant(P>0.05);the increasing concentration of Hcy increased MDA and NF-κB contents as well as the mRNA and protein expressions of TLR4

but it reduced the SOD contents.The activity of 100

200 μmol/L and 500 μmol/L Hcy compared to control cells was significant(P<0.05)

but other cells compared to control cells were not significant(P>0.05).Conclusion:The TLR4/NF-κB may act as one of important mechanisms in Hcy to promote the damage of HUVECs

in which MDA and SOD may play an important role.

关键词

人脐静脉内皮细胞动脉粥样硬化同型半胱氨酸Toll样受体4核因子-κB

Keywords

human umbilical vein endothelial cells(HUVECs)atherosclerosishomocysteinetoll-like receptor-4(TLR4)NF-κB

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