Study on the expression of MGMT gene in glioma stem cells derived from GBM

XU Zhong-ye1; CHEN Song1; CHENG Yuan1; WANG Zhi-gang2

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Study on the expression of MGMT gene in glioma stem cells derived from GBM

更新时间：2024-01-17

- Study on the expression of MGMT gene in glioma stem cells derived from GBM
- Journal of Chongqing Medical University Vol. 36, Issue 10, Pages: 1165-1167(2011)
- 作者机构：
  
  1. 重庆医科大学附属第二医院神经外科
  2. 重庆医科大学超声影像学研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC： R739.41
- Published：2011，
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[1]徐忠烨,陈松,程远,王志刚.多药耐药基因MGMT在脑胶质瘤干细胞中表达的实验研究[J].重庆医科大学学报,2011,36(10):1165-1167.

XU Zhong-ye1, CHEN Song1, CHENG Yuan1, et al. Study on the expression of MGMT gene in glioma stem cells derived from GBM. [J]. Journal of Chongqing Medical University 36(10):1165-1167(2011)
[1]徐忠烨,陈松,程远,王志刚.多药耐药基因MGMT在脑胶质瘤干细胞中表达的实验研究[J].重庆医科大学学报,2011,36(10):1165-1167. DOI：

XU Zhong-ye1, CHEN Song1, CHENG Yuan1, et al. Study on the expression of MGMT gene in glioma stem cells derived from GBM. [J]. Journal of Chongqing Medical University 36(10):1165-1167(2011) DOI：

摘要

目的:探讨甲基鸟嘌呤DNA甲基转移酶（Methylguanine-dna methyltransferase

MGMT）在脑胶质瘤细胞和胶质瘤干细胞（Brain glioma stem cells

BGSC）中的表达情况。方法:由手术切除的多形性胶质母细胞瘤中分离培养胶质瘤细胞和BGSC;应用免疫细胞化学染色和流式细胞术（Flow cytometry

FCW）鉴定BGSC;应用RT-PCR法测定MGMT mRNA在胶质瘤细胞、BGSC和正常脑组织中的表达。结果:本研究中获得的BGSC具有典型的BGSCs细胞生物学特征和免疫学标记;RT-PCR检测结果表明MGMT mRNA在BGSC中的相对表达量明显高于胶质瘤细胞（0.562±0.021 vs 0.112±0.023

P<0.01）

而胶质瘤细胞MGMTmRNA的相对表达量明显高于正常脑组织（0.112±0.023 vs 0.005±0.001

P<0.01）。结论:多药耐药基因MGMT主要在BGSC而非胶质瘤细胞中表达

其可能是BGSC产生多药耐药性的主要分子机制之一。

Abstract

Objective:To explore the expression of MGMT gene in glioma stem cells and glioma cells derived from glioblastoma.Methods:BGSC was isolated from glioblastoma and cultured in vitro

and was identified by immunocytochemical stain and flow cytometry.Relative expression of MGMT gene in BGSC and in glioma cells by RT-PCR was analyzed.Results:BGSC from glioblastoma could express Nestin and CD133 antigen

self-renew and differentiate into astrocyte and neuron in vitro.RT-PCR results demonstrated the relative expression of MGMT gene in BGSC was markedly higher than in glioma cells(0.562±0.021 vs 0.112±0.023

P<0.01)

and the relative expression of MGMT gene in glioma cells was markedly higher than in normal brain tissue(0.112±0.023 vs 0.005±0.001

P<0.01).Conclusion:The expression of MGMT gene in BGSC is markedly higher than in glioma cells.MGMT may be the key molecular biology mechanism that leads to drug resistance in BGSC.

关键词

胶质瘤干细胞胶质瘤细胞甲基鸟嘌呤DNA甲基转移酶

Keywords

brain glioma stem cellsglioma cellsmethylguanine-DNA methyltransferase

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