Influence of glucose on biodegradable waterborne polyurethane blend together with LL-37 in inhibiting ESBLs producing Escherichia coli biofilm in vitro

An Yu; Xiong Guobing; Qiu Mingxing; Wang Kunjie; Li Hong

您当前的位置：

首页 >

文章列表页 >

Influence of glucose on biodegradable waterborne polyurethane blend together with LL-37 in inhibiting ESBLs producing Escherichia coli biofilm in vitro

更新时间：2023-12-22

- Influence of glucose on biodegradable waterborne polyurethane blend together with LL-37 in inhibiting ESBLs producing Escherichia coli biofilm in vitro
- Journal of Chongqing Medical University Vol. 43, Issue 4, Pages: 482(2018)
- 作者机构：
  
  1. 四川省医学科学院、四川省人民医院泌尿外科,成都,610072
  2. 四川大学华西医院泌尿外科,成都,610041
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2018，
扫描看全文
An Yu, Xiong Guobing, Qiu Mingxing, et al. Influence of glucose on biodegradable waterborne polyurethane blend together with LL-37 in inhibiting ESBLs producing Escherichia coli biofilm in vitro. [J]. Journal of Chongqing Medical University 43(4):482(2018)
DOI：

An Yu, Xiong Guobing, Qiu Mingxing, et al. Influence of glucose on biodegradable waterborne polyurethane blend together with LL-37 in inhibiting ESBLs producing Escherichia coli biofilm in vitro. [J]. Journal of Chongqing Medical University 43(4):482(2018) DOI：

摘要

目的：探讨葡萄糖浓度变化对水性可降解聚氨酯（biodegradable waterborne polyurethane，BWPU）装载人源抗菌肽LL-37体外抑制ESBLs大肠埃希菌（Escherichia coli，E.coli）生物膜活性的影响。方法：采用尿源性ESBLs E.coli临床株（E44）为实验株，E.coli标准菌株ATCC25922（E0）做质控。构建胱细菌生物膜动态孵育模拟系统。“两步法”制备新型LDI－ＢＷＰＵ乳液PCLPU33。“物理溶解和常温干燥法”制备ＬＤＩ－ＢＷＰＵ装载不同LL-37浓度的载肽膜。设立4个实验分组：H组：高肽组（LL-37，2 000 ?滋g/mL）、L组：低肽组（LL-37，250 ?滋g/mL）、P组：阳性对照（亚胺培南，8 ?滋g/mL）、N组：阴性对照（无抗菌药），持续感染人工尿液环境下孵育48 h，观察不同葡萄糖浓度（0、11.1、33.3 mmol/L）及控制葡萄糖浓度（33.3→0 mmol/L）对载肽膜抑制生物膜的影响。检测方法包括：生物膜细菌活菌计数；Syto-9/PI荧光染色结合激光共聚焦显微镜构建立体图测量生物膜厚度；扫描电镜观察生物膜结构的细微观。生物膜细菌活菌计数和生物膜厚度的多组间均数比较采用单因素方差分析，α=0.01。结果：基于胱细菌生物膜动态孵育模拟系统，在持续感染人工尿液环境下不同葡萄糖浓度观测点，P、H、L组生物膜活菌计数和厚度均明显低于N组（aP=0.000）。H组生物膜活菌计数在无糖环境（G0 mmol/L）明显低于L组（bP=0.000），在含糖环境下（G11.1，33.3 mmol/L）与L组差异无统计学意义（P＞0.01）；H组生物膜厚度在不同葡萄糖观测点均明显低于L组（bP=0.000）。与无糖环境（G0 mmol/L）相比，含糖环境（G11.1，33.3 mmol/L）H、L组生物膜活菌计数和生物膜厚度均明显增加（cP=0.000）。调控葡萄糖浓度后（33.3→0 mmol/L），H、L组BF细菌活菌计数和生物膜厚度均明显降低（dP=0.000）。结论：持续感染人工尿液环境下不同葡萄糖浓度时，载肽膜通过抑制生物膜生长、杀灭生物膜细菌明显抑制细菌生物膜形成。

Abstract

Objective：To investigate the influence of the change of glucose concentration on ‘LL-37 sustained-release BWPU mem－brane’ in inhibiting the formation of the BF of urinary pathogenic ESBLs producing E.coli. Methods：Urinary pathogenic ESBLs pro－ducing E.coli isolates（E44） and standard strains ATCC25922（E0） were collected. Dynamic simulated bladder BF model was adopted. ‘Two-step approach’ was adopted to prepare new LDI-BWPU emulsion PCLPU33. ‘Physical dissolution and air-drying’ loading technique was adopted to prepare LL-37 sustained-release BWPU membrane.H group：high peptide（LL-37，2000 ?滋g/mL），L group：low peptide group（LL-37，250 ?滋g/mL），P group：positive control（Imipenem，8 ?滋g/mL），and N group：negative control（without antibacterial drug） were set up to incubate under artificial urine environment for 48hours. The influence of different glucose concen－trations（0，11.1，33.3 mmol/L），control of glucose concentration（33.3→0 mmol/L） on LL-37 sustained-release BWPU membrane in inhibiting BF were observed. The detection methods included viable bacteria count method. Syto-9/P fluorescent staining，combined with laser scanning confocal microscope，was applied to construct a space diagram to measure the BF thickness. Scan－ning electron microscope was adopted to observe the microscopic view of BF structure. One-way analysis of variance（ANOVA） was adopted in viable bacteria count，BF thickness and comparison of means of multiple groups. Results：For the observation point of different glucose concentration under the constantly infected artificial urine environment，the viable bacteria count and BF thickness of P group，H group，and L group were significantly decreased than that of N group（aP=0.000）. The viable bacteria count of group H was significantly decreased than that of L group（bP=0.000）in the glucose-free environment（G0 mmol/L）

and there was no statistical dif－ference between the H and L group in the glucose-containing environment（G11.1，33.3 mmol/L）（P＞0.01）；The BF thickness of the H group was significantly decreased than that of L group at the observation point of different glucose concentration（bP=0.000）. With the increase of glucose concentration，the viable bacteria count and BF thickness of the H and L groups，increased significantly（cP=0.000）. When glucose concentration was controlled（33.3→0 mmol/L） in the H and L groups，the viable bacteria count and BF thickness were were significantly decreased（dP=0.000）. Conclusion：When the glucose concentration is different under the constantly infected artificial urine environment，LL-37 sustained-release BWPU membrane can inhibit BF growth，kill BF bacteria and signifi－cantly inhibit BF formation.

关键词

β-内酰胺酶大肠埃希菌细菌生物膜葡萄糖可降解水性聚氨酯抗菌肽LL-37

Keywords

β-lactamasesEscherichia colibacterial biofilmglucosebiodegradablewaterborne polyurethaneantimicrobial peptideLL-37

references

Views

137

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Simulating the human bladder to construct a bacterial biofilm dynamic incubation system under biomimetic urinary flow stress in vitro

Applications and challenges of wearable sensing technology in laboratory medicine

Detection of plasmid-mediated AmpC expressed in Escherichia coli and Klebsiella pneumoniae by cefoxitin three-dimensional test

Construction of eukaryotic expression vector encoding antimicrobial peptides PR-39 and its expression in 293T cell

Influence of different glucose concentrations on the apoptosis of MC3T3-E1 osteoblast

Related Author

An Yu

Qiu Mingxing

Liu Jing

Huang Runhua

Wang Yu

Wang Shize

Taiju Chen

Hongjie Liu

Related Institution

Department of Urology，Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital

Center of Smart Laboratory and Molecular Medicine，School of Medicine，Chongqing University

Chongqing First Experimental Middle Secondary School

Laboratory Medicine Center，The Second Hospital of Shandong University

Clinical Laboratory, College of Clinical Medicine, Chongqing Medical University

⁰