Objective：To assess the prevalence of human immunodeficiency virus（HIV），risky sexual behaviors and the influencing factors of multiple sex partners（≥2 partners） in last 6 months among male students who have sex with men（MSM） in Chongqing and Sichuan. Methods：We adopted non-probability sampling method to recruited students MSM，and 200 students MSM were recruited to finish the survey and field HIV testing. Frequency was used to analyze the prevalence of HIV and logistic regression was used to analyze the influencing factors of multiple sex partners（≥2 partners） in last 6 months among student MSM. Results：There were 200 questionnaires and 193（96.50%） valid questionnaires. HIV antibody positive rate was 8.29%，the HIV antibody posi－tive detection rate of student who studied at secondary school（including high school，technical secondary school and professional high school），junior college and university and above were 17.95%，8.33% and 5.08%，respectively. The HIV antibody positive detection rate whose registered permanent residence was rural and not-receiving HIV testing were 12.70% and 11.11%，respectively. In the last month，82.47%（127 persons） did not use condom ev－ery time when they had sexual intercourse. In the last 6 months，51.81%（100 persons） had multiple sexual partners（≥2 partners）. Logistic regression showed that contributing factors of multiple sex partners（≥2 partners） in last 6 months were student who studied at junior college（OR=4.270，P=0.0083），student who studied at University and above（OR=3.523，P=0.0043），having male sex partner via internet in last 6 months（OR=5.622，P<0.0001），and having unprotected sex intercourse in last 1 month（OR=2.255，P=0.0201）. Conclusion：In Chongqing and Sichuan，the prevalence of HIV infection among secondary school and college students MSM is high，especially among secondary school students MSM and students whose registered permanent residence is rural. And also in Chongqing and Sichuan，high-risk sexual behavior and having had multiple sex partners are still commonly seen among students MSM students. Interventions are needed to prevent HIV transmission in this population．