Objective：To establish a modified mouse kidney transplantation model with simple operation，short operation time and exact effect. Methods：The end to end anastomosis was perform on the veins，the donor kidney vena cava was evaginated after inserting catheter，and the evaginated vena cava was put into the receptor renal vein like sleeve. The abdominal aorta of the donor was anasto－mosed end-to-side to the abdominal aorta of the recipient. The donor bladder patch was anastomosed with the recipient bladder. Results：After open the blood vessels，the renal color was normal，the arterial pulsation was normal，the vein filling was good，and the kidney transplantation model of the mouse was successfully established. The venous anastomosis was （10±5） min，the arterial anas－tomosis was （10±5） min，and the bladder flap anastomosed was 2-5 min. Total operation time was 40-60 min. Conclusion：Using the modified type of venous anastomosis and the anastomosis of the bladder flap can reduce the difficulty and shorten the operation time. The mouse kidney transplantation model could be established successfully.