Silencing PRR11 inhibits the migration and invasion of lung cancer A549 cells

Zhang Lian; Zhang Chundong; Bu Youquan; Song Fangzhou

您当前的位置：

首页 >

文章列表页 >

Silencing PRR11 inhibits the migration and invasion of lung cancer A549 cells

更新时间：2023-12-22

- Silencing PRR11 inhibits the migration and invasion of lung cancer A549 cells
- Journal of Chongqing Medical University Vol. 43, Issue 5, Pages: 662(2018)
- 作者机构：
  
  重庆医科大学生物化学与分子生物学教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2018，
扫描看全文
Zhang Lian, Zhang Chundong, Bu Youquan, et al. Silencing PRR11 inhibits the migration and invasion of lung cancer A549 cells. [J]. Journal of Chongqing Medical University 43(5):662(2018)
DOI：

Zhang Lian, Zhang Chundong, Bu Youquan, et al. Silencing PRR11 inhibits the migration and invasion of lung cancer A549 cells. [J]. Journal of Chongqing Medical University 43(5):662(2018) DOI：

摘要

目的：PRR11（proline rich protein 11）是近年来发现的一个肿瘤相关基因，前期研究结果显示其高表达对于肺癌等恶性肿瘤细胞的侵袭迁移具有重要作用，本研究为了更进一步研究其促进肺癌细胞侵袭转移的分子机制。方法：综合利用siRNA敲减、划痕及迁移实验、Western blot及免疫荧光等方法分析人肺癌细胞系A549沉默PRR11表达后细胞侵袭迁移能力下降的分子机制。结果：PRR11沉默后，划痕及Transwell实验结果表明，PRR11沉默明显减弱了A549细胞运动、侵袭及迁移能力。进一步定量qRT-PCR及Western blot检测发现，PRR11稳定沉默导致上皮细胞标志分子E-cadherin明显上调、而EMT上游关键转录因子Twist1及间质细胞标志分子N-cadherin表达下调，另外免疫荧光染色结果同样显示Twist1及N-cadherin下调明显。结论：肺癌细胞系A549中PRR11可能通过调节Twist1等EMT关键分子的表达在细胞侵袭转移过程中起关键作用，为进一步探索PRR11在肺癌中的作用机制奠定了基础。

Abstract

Objective：To further investigate the molecular mechanism that proline rich protein 11（PRR11） implicates in migration and invasion in lung cancercells. Methods：PRR11 was down-regulated using siRNA in lung cancer cell A549. The migration and inva－sion abilities was detected using wound healing，Transwell，siRNA，and EMT relative proteins was analyzed by Western blot and IF assay. Results：Our results demonstrated that PRR11 depletion significantly inhibited the migration and invasion abilities in A549 cells using wound healing and Transwell. Furthermore，the expression levels of the Twist1 and N-cadherin were down-regulated in PRR11 depletion significantly than that of control using qRT-PCR，Western blot and IF. Conclusion：PRR11 depletion significantly inhibites the migration and invasion abilities in lung cancer cell line. It suggests that high-level expression of PRR11 enhance the migration and invasion activities in lung cancer，but the molecular mechanism is worth for further study.

关键词

肺癌侵袭迁移PRR11Twist1

Keywords

lung cancermigrationinvasionproline rich protein 11Twist1

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Glycyrrhiza uralensis Fisch. endophytes combined with cisplatin on proliferation and apoptosis of A549 cells

Establishment and validation prediction models of benign and malignant predictors for solitary pulmonary nodules

Clinical analysis of dermatomyositis/polymyositis complicated with lung disease

Effect of progressive muscle relaxation training on coping style and emotion of patients with lung cancer

Related Author

Zhang Shanglong

Zhang Nan

Lian Xiaolong

Ye Liqiao

Ma Quhuan

Deng Yi

Zhu Xiaoqian

Zheng Li

Related Institution

College of Pharmacy，Gansu University of Chinese Medicine

Department of Respiratory and Critical Care Medicine, Chongqing Medical University

Department of Respiratory,The First Affiliated Hospital of Chongqing Medical University

Department of Clinic Nursing,Xiangya Nursing School of Central South University

Department of Respiratory,the Second Affiliated Hospital,Chongqing Medical University

⁰