Objective：To evaluate the safety and efficacy concomitant atrial fibrillation ablation for patients undergoing mitral surgery via mini-thoracotomy with thorascopic assistance. Methods：From March 1st 2016 to May 10th 2017，41 patients undergoing atrial fibril－lation ablation with valve surgery in Chongqing General Hospital were enrolled and divided into two groups. Two groups were divided by the operational approach. A group were the patients underwent radiofrequecy mazeⅢ procedure during mini-invasive valve surgery. B groups were the patients underwent radiofrequecy mazeⅢ procedure with valve surgery by median sternotomy. The rate of freedom from atrial fibrillation（after procedure：76.9% vs. 78.5%，P=1.000，10 days later：84.6% vs. 78.5%，P=0.975），the time of ablation pro－cedure[（41.9±4.0） min vs. （39.7±5.4） min，P=0.198]，the diameter of left atrium[（47.6±6.3） mm vs. （49.5±6.6） mm，P=0.384]，the volume of chest drainage[（342.9±44.3） mL vs. （47.1±132.5） mL，P=0.020]，the length of the incision[（5.1±0.6） cm vs. （20.7±2.6） cm，P=0.000] were compared between two groups. Results：Compared with B groups，A groups has no difference in the rate of freedom from atial fibrillation；no difference in the time of ablation procedure and the diameter of left atrium was observed. However，there were significant differences in the volume of chest drainage（P=0.020），the length of the incision（P=0.000）. Conclusion：The concomitant atrial fibrillation ablation for patients undergoing mitral surgery via mini-thoracotomy with thorascopic assistance is safe and feasible.