Objective：To investigate the clinical effect of CO2 cryotherapy in the treatment of cervical lesions，including chronic cer－vicitis and cervical intraepithelial neoplasia（CIN），and related influencing factors. Methods：A total of 129 patients who underwent CO2 cryotherapy for cervical lesions in Department of Obstetrics and Gynecology in The Second Affiliated Hospital of Chongqing Medical University from April 2016 to July 2017 and had complete follow-up data were enrolled，and a retrospective analysis was performed for treatment outcome，complications，and influencing factors. Results：The overall response rate of cervical CO2 cryotherapy was 80.6%（104/129）；the patients with chronic cervicitis had a response rate of 86.1%（62/72），and those with CIN had a response rate of 73.7%（42/57）. The patients with cervical lesions and human papillomavirus（HPV） infection had an HPV remission rate of 63.0%（51/81）. Conclusion：CO2 cryotherapy has a marked clinical effect in the treatment of chronic cervicitis，CIN，and HPV infection and thus holds promise for clinical application.