Objective：To investigate differentially expressed microRNAs between different lung cell lines under hypoxic conditions，and to provide a reference for further research on the mechanism of action of microRNAs in lung cancer cells under hypoxia. Methods：A model of hypoxia was established. RNA was extracted from GLC-82 and A549 cells cultured under a hypoxic or normal oxygen concentration，and microarray assay and PCR were used to screen out differentially expressed microRNAs. TargetScanHuman 7.2 and cytoscape software were used to predict the targeted genes regulated by differentially expressed microRNAs and the signaling path－ways involving these target genes. Results：Under the hypoxic condition，3 microRNAs were upregulated and 30 were downregulated in A549 cells，while 2 microRNAs were upregulated and 31 were downregulated in GLC-82 cells. miR-192-5p showed an opposite change trend in these two cell lines，while the other microRNAs showed a similar change trend. Kyoto Encyclopedia of Genes and Genome and gene ontology analyses showed that the differ－entially expressed microRNAs were mainly involved in tumor-related signaling pathways，insulin signaling pathways，Ras sig－naling pathways，and oxygen metabolism. Conclusion：Differentially expressed microRNAs are found in lung cancer cell lines cultured under hypoxic or normal oxygen conditions，and there are also differentially expressed microRNAs between cells with different genetic backgrounds. The target genes of differentially expressed microRNAs are involved in a variety of cellular metabolic pathways and have the functions of oxygen metabolism and oxygen response.