Objective: To investigate the effect of silencing PARG on the proliferation and differentiation of tumor local B220+DEC205+DC and its role in colon carcinoma metastasis. Methods：Experiment group was established by transfecting lentivirus Poly (ADP-ribose) glycohydrolase short hairpin RNA (PARG-shRNA) into mouse colon carcinoma CT26 cells and control group by enrolling CT26 cells without any treatment and CT26 cells with empty vector.Liver metastasis model of colon carcinoma was established by splenic subcapsular inoculation.The expressions of PARG

PARP and NF-κB proteins in splenic carcinoma were detected by Western blot and those of B220+DEC205+DC in the spleen by immunofluorescence double labeling assay.The levels of IL-10 and TGF-β in the serum were measured by enzyme-linked immunosorbent assay(ELISA). Results：The size of splenic carcinoma and the number of metastatic liver nodules in experiment group were smaller and lower than those in control group (P<0.05).The expressions of PARG

PARP and NF-κB in splenic carcinoma were reduced obviously in experiment group compared with those in control group (P<0.05).The number of B220+DEC205+DC in the spleen was significantly fewer in experiment group than those in control group (P<0.05) and the levels of IL-10 and TGF-β in the serum were significantly decreased in experiment group than those in control group (P<0.05). Conclusion：These studies demonstrate that silencing PARG could suppress the metastasis of CT26 cells to the liver

which is possibly related with the processes of down regulating of both PARP and NF-κB as well as decreasing IL-10 and TGF-β and finally affecting the proliferation and differentiation of local B220+DEC205+DC.